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Titolo:
Vascular corrosion casting: Review of advantages and limitations in the application of some simple quantitative methods
Autore:
Hossler, FE; Douglas, JE;
Indirizzi:
E Tennessee State Univ, James H Quillen Coll Med, Dept Anat & Cell Biol, Johnson City, TN 37614 USA E Tennessee State Univ Johnson City TN USA 37614hnson City, TN 37614 USA E Tennessee State Univ, James H Quillen Coll Med, Dept Internal Med, Johnson City, TN 37614 USA E Tennessee State Univ Johnson City TN USA 37614 hnson City, TN 37614 USA
Titolo Testata:
MICROSCOPY AND MICROANALYSIS
fascicolo: 3, volume: 7, anno: 2001,
pagine: 253 - 264
SICI:
1431-9276(200105/06)7:3<253:VCCROA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCANNING ELECTRON-MICROSCOPY; ACCESSORY RESPIRATORY ORGANS; 3-DIMENSIONAL MORPHOMETRY; CAPILLARY DIAMETER; RAT-HEART; MICROCIRCULATION; PATTERN; SPHINCTERS; MYOCARDIUM; TADPOLES;
Keywords:
vascular corrosion casting; microvasculature; endothelial cells; scanning electron microscopy; venous valves; heart; lung; salt gland; emphysema;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Hossler, FE E Tennessee State Univ, James H Quillen Coll Med, Dept Anat & Cell Biol, Box 70582, Johnson City, TN 37614 USA E Tennessee State Univ Box70582 Johnson City TN USA 37614 USA
Citazione:
F.E. Hossler e J.E. Douglas, "Vascular corrosion casting: Review of advantages and limitations in the application of some simple quantitative methods", MICROS MICR, 7(3), 2001, pp. 253-264

Abstract

Vascular corrosion casting has been used for about 40 years to produce replicas of normal and abnormal vasculature and microvasculature of various tissues and organs that could be viewed at the ultra-structural level. In combination with scanning electron microscopy (SEM), the primary application of corrosion casting has been to describe the morphology and anatomical distribution of blood vessels in these tissues. However, such replicas should also contain quantitative information about that vasculature. This report summarizes some simple quantitative applications of vascular corrosion casting. Casts were prepared by infusing Mercer resin or diluted Mercer resin into the vasculature. Surrounding tissues were removed with KOH, hot water, and formic acid, and the resulting dried casts were observed with routine SEM, The orientation, size, and frequency of vascular endothelial cells were determined from endothelial nuclear imprints on various cast surfaces. Vascular volumes of heart, lung, and avian salt gland were calculated using tissue and resin densities, and weights. Changes in vascular volume and functional capillary density in an experimentally induced emphysema model were estimated from confocal images of casts. Clearly, corrosion casts lend themselves to quantitative analysis. However, because blood vessels differ in their compliances, in their responses to the toxicity of casting resins, and intheir response to varying conditions of corrosion casting procedures, it is prudent to use care in interpreting this quantitative data. Some of the applications and limitations of quantitative methodology with corrosion casts are reviewed here.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:47:04