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Titolo:
Role of glucocorticoids in the maturation of the rat renal Na+/H+ antiporter (NHE3)
Autore:
Gupta, N; Tarif, SR; Seikaly, M; Baum, M;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Pediat & Internal Med, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 ediat & Internal Med, Dallas, TX 75390 USA
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 1, volume: 60, anno: 2001,
pagine: 173 - 181
SICI:
0085-2538(200107)60:1<173:ROGITM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; BORDER MEMBRANE-VESICLES; 11-BETA-HYDROXYSTEROID DEHYDROGENASE SYSTEM; BETA-HYDROXYSTEROID DEHYDROGENASE; TUMOR VIRUS GLYCOPROTEINS; BRUSH-BORDER; MOLECULAR-CLONING; EXCHANGER ISOFORM; MESSENGER-RNA; TRANSLATIONAL REGULATION;
Keywords:
NHE3; adrenalectomy; renal development; microperfusion; cell pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Baum, M Univ Texas, SW Med Ctr, Dept Pediat, 5323 Harry Hines Blvd, Dallas, TX 75390 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75390 TX 75390 USA
Citazione:
N. Gupta et al., "Role of glucocorticoids in the maturation of the rat renal Na+/H+ antiporter (NHE3)", KIDNEY INT, 60(1), 2001, pp. 173-181

Abstract

Background. Neonates have a lower Na+/H+ antiporter activity on the apicalmembrane of proximal tubule than that of adults. The maturational increasein Na+/H+ antiporter activity occurs at the time when there is a rise in serum glucocorticoid levels in rats. The purpose of the present study was toexamine whether glucocorticoids are responsible for the postnatal increasein Na+/H+ antiporter activity. Methods. Nine-day-old Sprague-Dawley rats were compared with rats studied at 30 days of age who had either a sham operation or adrenalectomy (ADX) atnine days of age and with rats that had an adrenalectomy and physiologic corticosterone replacement (ADX-Cort) to determine whether glucocorticoid deficiency prevented the maturational increase in Na+/H+ antiporter activity. Na+/H+ antiporter activity was measured in proximal convoluted tubules perfused in vitro by the change in cell pH (pH,) following luminal sodium removal. NHE3 mRNA abundance was measured using Northern blot analysis, and NHE3 protein abundance was measured by immunoblot. Results. Na+/H-divided by antiporter activity was 93.8 +/- 17.7, 157.0 +/-18.0, 356.7 +/- 29.9, and 102.5 +/- 14.5 pmol/mm min in nine-day-old, ADX. ADX-Cort, and sham control groups. respectively. The ADX-Cort and sham control were higher than the 9-day-old and the 30-day-old ADX group (P < 0.05). Brush-border membrane NHE3 protein abundance in the nine-day-old and ADX groups were sixfold less than ADX-Cort and sham control groups (P < 0.001). Nine-day-old neonates had fivefold less renal cortical NHE3 mRNA than the ADX, ADX-Cort, and sham-operatrd control groups (P < 0.01). Conclusions. These data demonstrate that glucocorticoids play a role in the postnatal maturation of the proximal tubule Na+/H+ antiporter activity and brush-border membrane NHE3 protein abundance. Glucocorticoid deficiency does not completely prevent the maturational increase in Na+/H+ antiporter activity and does not affect NHE3 mRNA abundance.

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Documento generato il 04/04/20 alle ore 02:34:13