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Titolo:
High glucose induces MCP-1 expression partly via tyrosine kinase-AP-1 pathway in peritoneal mesothelial cells
Autore:
Lee, SK; Kim, BS; Yang, WS; Kim, SB; Park, SK; Park, JS;
Indirizzi:
Univ Ulsan, Coll Med, Dept Internal Med, Seoul, South Korea Univ Ulsan Seoul South Korea Med, Dept Internal Med, Seoul, South Korea Univ Ulsan, Coll Med, Dept Gen Surg, Seoul, South Korea Univ Ulsan SeoulSouth Korea oll Med, Dept Gen Surg, Seoul, South Korea Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul, South Korea Univ Ulsan Seoul South Korea ed, Asan Inst Life Sci, Seoul, South Korea
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 1, volume: 60, anno: 2001,
pagine: 55 - 64
SICI:
0085-2538(200107)60:1<55:HGIMEP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT PROTEIN-1; GLYCATION END-PRODUCTS; HUMAN MESANGIAL CELLS; NF-KAPPA-B; GENE-EXPRESSION; CHEMOTACTIC PROTEIN-1; DIALYSIS PATIENTS; BINDING; ACTIVATION; IDENTIFICATION;
Keywords:
CAPD; dialysate; fibrosis; monocyte chemoattractant protein-1; activator protein-1; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, SK Asan Med Ctr, Dept Internal Med, Div Nephrol, Songpa Ku, 388-1 Poongnap Dong, Seoul 138736, South Korea Asan Med Ctr 388-1 Poongnap Dong Seoul South Korea 138736 h Korea
Citazione:
S.K. Lee et al., "High glucose induces MCP-1 expression partly via tyrosine kinase-AP-1 pathway in peritoneal mesothelial cells", KIDNEY INT, 60(1), 2001, pp. 55-64

Abstract

Background. High glucose in peritoneal dialysis solutions has been implicated in the pathogenesis of peritoneal fibrosis in chronic ambulatory peritoneal dialysis (CAPD) patients. However. the mechanisms are not very clear. Peritoneal macrophages seem to participate in the process of peritoneal fibrosis and monocyte chemoattractant protein-1 (MCP-I) plays a key role in the recruitment of monocytes toward the peritoneal cavity. However. little isknown about the effect of high glucose on MCP-1 expression and its signal transduction pathway in human peritoneal mesothelial cells. Methods. Mesothelial cells were cultured with glucose (5 to 100 mmol/L) ormannitol chronically for up to seven days. MCP-1 expression of mRNA and protein was measured by Northern blot analysis and enzyme-linked immunosorbent assay (ELISA). Chemotactic activity of high-glucose-conditioned culture supernatant was measured by chemotactic assay. To examine the roles of the transcription factors activator protein-1 (AP-1) and nuclear factor-KB (NF-kappaB), electrophoretic mobility shift assay (EMSA) was performed. Results. Glucose induced MCP-1 mRNA expression in a time-and dose-dependent manner. MCP-1 protein in cell culture supernant was also increased. Equivalent concentrations of mannitol had no significant effect. High-glucose-conditioned supernatant possessed an increased chemotactic activity for monocytes, which was neutralized by anti-MCP-1 antibody. EMSA revealed that glucose increased the AP-1 binding activity in a time- and dose-dependent manner. but not NF-kappaB. Curcumin, an inhibitor of AP-I, dose-dependently suppressed the induction of MCP-I mRNA by high glucose. Tyrosine kinase inhibitors such as genistein (12.5 to 50 mu mol/L) and herbimycin A (0.1 to I mu mol/L) inhibited the high-glucose-induced MCP-I mRNA expression in a dose-dependent manner. and also suppressed the high-glucose-induced AP-1 binding activity. Conclusions. High glucose induced mesothelial MCP-1 expression partly via the tyrosine kinase-AP-l pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:41:15