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Titolo:
VEGF AND VEGF-C - SPECIFIC INDUCTION OF ANGIOGENESIS AND LYMPHANGIOGENESIS IN THE DIFFERENTIATED AVIAN CHORIOALLANTOIC MEMBRANE
Autore:
OH SJ; JELTSCH MM; BIRKENHAGER R; MCCARTHY JEG; WEICH HA; CHRIST B; ALITALO K; WILTING J;
Indirizzi:
UNIV FREIBURG,ANATOM INST 2,ALBERTSTR 17 D-79104 FREIBURG GERMANY UNIV HELSINKI,HAARTMAN INST,MOL CANC BIOL LAB FIN-00014 HELSINKI FINLAND NATL BIOL RES CTR,DEPT GENE EXPRESS D-38124 BRAUNSCHWEIG GERMANY CATHOLIC UNIV,COLL MED,DEPT ANAT SEOUL 137701 SOUTH KOREA
Titolo Testata:
Developmental biology
fascicolo: 1, volume: 188, anno: 1997,
pagine: 96 - 109
SICI:
0012-1606(1997)188:1<96:VAV-SI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; VASCULAR-PERMEABILITY FACTOR; LYMPHATIC CAPILLARIES; MONOCLONAL-ANTIBODY; TYROSINE KINASE; BLOOD-VESSEL; CELL GROWTH; IN-VIVO; PLACENTA; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Oh et al., "VEGF AND VEGF-C - SPECIFIC INDUCTION OF ANGIOGENESIS AND LYMPHANGIOGENESIS IN THE DIFFERENTIATED AVIAN CHORIOALLANTOIC MEMBRANE", Developmental biology, 188(1), 1997, pp. 96-109

Abstract

The lymphangiogenic potency of endothelial growth factors has not been studied to date. This is partially due to the lack of in vivo lymphangiogenesis assays. We have studied the lymphatics of differentiated avian chorioallantoic membrane (CAM) using microinjection of Mercer resin, semi- and ultrathin sectioning, immunohistochemical detection of fibronectin and alpha-smooth muscle actin, and in situ hybridization with VEGFR-2 and VEGFR-3 probes, CAM is drained by lymphatic vessels which are arranged in a regular pattern. Arterioles and arteries are accompanied by a pair of interconnected lymphatics and form a plexus around bigger arteries. Veins are also associated with lymphatics, particularly larger veins, which are surrounded by a lymphatic plexus. The lymphatics are characterized by an extremely thin endothelial lining, pores, and the absence of a basal lamina, Patches of the extracellular matrix can be stained with an antibody against fibronectin. Lymphatic endothelial cells of differentiated CAM show ultrastructural features ofthis cell type. CAM lymphatics do not possess mediae. In contrast, the lymphatic trunks of the umbilical stalk are invested by a single butdiscontinuous layer of smooth muscle cells, CAM lymphatics express VEGFR-2 and VEGFR-3. Both the regular pattern and the typical structure of these lymphatics suggest that CAM is a suitable site to study the in vivo effects of potential lymphangiogenic factors. We have studied the effects of VEGF homo- and heterodimers, VEGF/PlGF heterodimers, andPlGF and VEGF-C homodimers on Day 13 CAM. All the growth factors containing at least one VEGF chain are angiogenic but do not induce lymphangiogenesis. PlGF-1 and PlGF-2 are neither angiogenic nor lymphangiogenic. VEGF-C is the first lymphangiogenic factor and seems to be highlychemoattractive for lymphatic endothelial cells. It induces proliferation of lymphatic endothelial cells and development of new lymphatic sinuses which are directed immediately beneath the chorionic epithelium. Our studies show that VEGF and VEGF-C are specific angiogenic and lymphangiogenic growth factors, respectively. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 12:27:13