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Titolo:
Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus
Autore:
Johansen, IE; Lund, OS; Hjulsager, CK; Laursen, J;
Indirizzi:
Danish Inst Agr Sci, Biotechnol Grp, DK-1871 Frederiksberg C, Denmark Danish Inst Agr Sci Frederiksberg Denmark C 871 Frederiksberg C, Denmark
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 14, volume: 75, anno: 2001,
pagine: 6609 - 6614
SICI:
0022-538X(200107)75:14<6609:RRIPSA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORNE MOSAIC-VIRUS; TOBACCO ETCH POTYVIRUS; BETA-GLUCURONIDASE; MOLECULAR-BIOLOGY; INFECTED CELLS; POTATO-VIRUS; RNA VIRUSES; IN-VIVO; PEA; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Johansen, IE Danish Inst Agr Sci, Biotechnol Grp, Thorvaldsensvej 10-4, DK-1871 Frederiksberg C, Denmark Danish Inst Agr Sci Thorvaldsensvej 10-4 Frederiksberg Denmark C
Citazione:
I.E. Johansen et al., "Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus", J VIROLOGY, 75(14), 2001, pp. 6609-6614

Abstract

Pea seed-borne mosaic potyvirus (PSbMV) isolates are divided into pathotypes P-1, P-2, and P-4 according to their infection profile on a panel of Pisum sativum lines. P. sativum PI 269818 is resistant to P-1 and P-2 isolatesand is susceptible to P-4 isolates. Resistance to P-1 is inherited as a single recessive gene, denoted sbm-1, and the pathogenicity determinant has previously been mapped to the virus-coded protein VPg. In the cultivar Bonneville, a second recessive gene, sbm-2, confers specific resistance to P-2. By exchanging cistrons between a P-2 and a P-4 isolate, the P3-6k1 cistron was identified as the PSbMV host-specific pathogenicity determinant on Bonneville. Exchange of P3-6k1 did not affect infection on PI 269818, and infection of Bonneville was not altered by substitution of the VPg cistron, indicating that P3-6k1 and VPg are independent determinants of pathotype-specific infectivity. On PI 269818 the pathogenicity determinant of both P-l and P-2 mapped to the N terminus of VPg. This suggests that VPg from the P-1 and P-2 isolates are functionally similar on this host and that resistance toP-1 and P-2 in PI 269818 may operate by the same mechanism. Identificationof VPg-sbm-1 and P3-6k1-sbm-2 as independent pairs of genetic interactors between PSbMV and P. sativum provides a simple explanation of the three known pathotypes of PSbMV. Furthermore, analysis of P-glucuronidase-tagged P-2virus indicated that sbm-2 resistance affected an early step in infection,implying that the P3-6k1 region plays a critical role in potyvirus replication or cell-to-cell movement.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:19:36