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Titolo:
The effect of combined arterial hemodynamics on saphenous venous endothelial nitric oxide production
Autore:
Casey, PJ; Dattilo, JB; Dai, GH; Albert, JA; Tsukurov, OI; Orkin, RW; Gertler, JP; Abbott, WM;
Indirizzi:
Massachusetts Gen Hosp, Div Vasc Surg, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 asc Surg, Boston, MA 02114 USA
Titolo Testata:
JOURNAL OF VASCULAR SURGERY
fascicolo: 6, volume: 33, anno: 2001,
pagine: 1199 - 1204
SICI:
0741-5214(200106)33:6<1199:TEOCAH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUTOGENOUS VEIN GRAFTS; FLUID SHEAR-STRESS; INTIMAL HYPERPLASIA; CYCLIC STRAIN; VASCULAR WALL; L-ARGININE; IN-VITRO; CELLS; PHYSIOLOGY; FLOW;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Abbott, WM Massachusetts Gen Hosp, Div Vasc Surg, 15 Parkman St,WAC 458, Boston, MA 02114 USA Massachusetts Gen Hosp 15 Parkman St,WAC 458 Boston MA USA 02114
Citazione:
P.J. Casey et al., "The effect of combined arterial hemodynamics on saphenous venous endothelial nitric oxide production", J VASC SURG, 33(6), 2001, pp. 1199-1204

Abstract

Introduction: Evidence exists that an ideal bypass conduit should have a functional endothelial cell surface combined with mechanical properties similar to those of native arteries. We hypothesized that the effect of combined arterial levels of pulsatile shear stress, flow, and cyclic strain would enhance saphenous venous endothelial cell nitric oxide (NO) production, andthat variations in these "ideal" conditions could impair this function. Westudied NO production as a measure of endothelial function in response to different hemodynamic conditions. Methods: Human adult saphenous venous endothelial cells were cultured in 10-cm silicone tubes, similar in diameter (5 mm) and compliance (6%) to a medium-caliber peripheral artery (eg, popliteal). Tube cultures were exposed to arterial conditions: a combined pressure (120/80 mm/Hg; mean, 100 mm/Hg), flow (mean, 115 mL/min) and cyclic strain (2%), with a resultant pulsatile shear stress of 4.8 to 9.4 dyne/cm(2) (mean, 7.1). Identical tube cultures were used to study variations in these conditions. Modifications of the system included a noncompliant system, a model with non-pulsatile flow, and a final group exposed to pulsatile pressure with no flow NO levels were measured with, fluorometric nitrite assay of conditioned media collected at 0,0.25, 0.5, 1, 2, and 4 hours. Experimental groups were compared with cellsexposed to nonpulsatile, nonpressurized low flow (shear stress 0.1 dyne/cm(2)) and static cultures. Results: Ah experimental groups had greater rates of NO production than cells under static conditions (P < .05). Cells exposed to ideal conditions produced the greatest levels of NO. Independent decreases in compliance, flow, and pulsatility resulted in significantly lower rates of NO production than those in the group with these conditions intact (vs noncompliant P < .05, vs nonflow P < .05, and vs nonpulsatile P < .05). Conclusions: Our results show that in the absence of physiologically normal pulsatility, cyclic strain, and Volume flow endothelial NO production does not reach the levels seen under ideal conditions. Pulsatile flow and compliance (producing flow with cyclic stretch) play a key role in NO production by vascular endothelium in a three-dimensional hemodynamically active model. This correlates biologically with clinical experience linking graft inflow and runoff and the mechanical properties of the conduit to long-term patency.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:09:53