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Titolo:
Altered signaling and regulatory mechanisms of apoptosis in focal and segmental glomerulosclerosis
Autore:
Wang, WS; Tzanidis, A; Divjak, M; Thomson, NM; Stein-Oakley, AN;
Indirizzi:
Monash Univ, Alfred Hosp, Sch Med, Dept Med, Prahran, Vic 3181, Australia Monash Univ Prahran Vic Australia 3181 Med, Prahran, Vic 3181, Australia
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 7, volume: 12, anno: 2001,
pagine: 1422 - 1433
SICI:
1046-6673(200107)12:7<1422:ASARMO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; EPITHELIAL-CELLS; MESANGIAL CELLS; CYTOCHROME-C; EXPRESSION; PROTEIN; BCL-2; SURVIVAL; PROLIFERATION; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Stein-Oakley, AN Monash Univ, Alfred Hosp, Sch Med, Dept Med, Prahran, Vic3181, Australia Monash Univ Prahran Vic Australia 3181 c 3181, Australia
Citazione:
W.S. Wang et al., "Altered signaling and regulatory mechanisms of apoptosis in focal and segmental glomerulosclerosis", J AM S NEPH, 12(7), 2001, pp. 1422-1433

Abstract

The purpose of this study was to investigate signaling and regulatory mechanisms of apoptosis in a model of focal and segmental glomerulosclerosis. Sprague-Dawley rats received two doses of puromycin aminonucleoside (PAN) (day 0 and week 3) and a uninephrectomy (PAN model). Apoptosis was detected with the use of the terminal deoxynucleotidyl transferase mediated dUTP nickend labeling technique. Bax, Bcl-2, Fas, and Fas ligand expression was analyzed by competitive reverse transcription-PCR. Bax, Bcl-2, and Fas mRNA were localized by in situ hybridization. Renal function was transiently impaired after the first PAN dose. After the second PAN dose, further progressive renal impairment, tubular atrophy, interstitial fibrosis, and glomerulosclerosis were evident. Eighteen percent of PAN samples demonstrated up to 4 apoptotic cells/50 glomeruli, compared with 7% of sham controls (not significant). No consistent significant change in glomerular Bax, Bcl-2, Fas, andFas ligand mRNA was evident by reverse transcription-PCR, although focal increases in glomerular Bcl-2 mRNA were demonstrated by in situ hybridization. In the tubulointerstitium, apoptosis was increased from weeks to 12 (P <0.01 PAN versus sham), correlated to renal function and tubulointerstitialinjury (P < 0.01). Total renal Bax, Fas, and Fas ligand mRNA were upregulated in the PAN model, peaking at week 17 (P < 0.01 versus sham), whereas Bcl-2 mRNA was not significantly different in PAN versus sham controls. In situ hybridization in the PAN model demonstrated prominent Bax mRNA in dilated tubules and infiltrating leukocytes. Fts mRNA signal was localized to tubular epithelial cells and leukocytes. The results suggest that altered apoptotic signaling and regulatory mechanisms contribute to the tubulointerstitial injury in this model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:46:15