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Titolo:
The potential of bone marrow-derived cells to differentiate to glomerular mesangial cells
Autore:
Imasawa, T; Utsunomiya, Y; Kawamura, T; Zhong, Y; Nagasawa, R; Okabe, M; Maruyama, N; Hosoya, T; Ohno, T;
Indirizzi:
Jikei Univ, Sch Med, Dept Internal Med, Tokyo, Japan Jikei Univ Tokyo Japan i Univ, Sch Med, Dept Internal Med, Tokyo, Japan Jikei Univ, Sch Med, Dept Microbiol, Tokyo, Japan Jikei Univ Tokyo Japan ikei Univ, Sch Med, Dept Microbiol, Tokyo, Japan Saitama Med Ctr, Saitama Med Sch, Dept Internal Med, Moroyama, Saitama, Japan Saitama Med Ctr Moroyama Saitama Japan nal Med, Moroyama, Saitama, Japan Osaka Univ, Microbial Dis Res Inst, Osaka, Japan Osaka Univ Osaka JapanOsaka Univ, Microbial Dis Res Inst, Osaka, Japan Tokyo Metropolitan Inst Gerontol, Dept Mol Pathol, Tokyo, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan t Mol Pathol, Tokyo, Japan
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 7, volume: 12, anno: 2001,
pagine: 1401 - 1409
SICI:
1046-6673(200107)12:7<1401:TPOBMC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESENCHYMAL STEM-CELLS; IGA NEPHROPATHY; IN-VITRO; MICE; KIDNEY; GLOMERULONEPHRITIS; DISORDER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Imasawa, T Tokai Univ, Sch Med, Inst Med Sci, Kanagawa 2591131, Japan Tokai Univ Kanagawa Japan 2591131 ci, Kanagawa 2591131, Japan
Citazione:
T. Imasawa et al., "The potential of bone marrow-derived cells to differentiate to glomerular mesangial cells", J AM S NEPH, 12(7), 2001, pp. 1401-1409

Abstract

Bone marrow stem cells (BMC) develop into hematopoietic and mesenchymal lineages but have not been known to differentiate into glomerular cells. To investigate whether such differentiation is possible, a search was made for donor glomerular cells in lethally irradiated C57BL/6j (B6) mice given transplants of BMC from syngeneic mice transgenic for green fluorescence protein (GFP) ([GFP-->B6] mice). After the recipients of donor BMC manifested GFP-positive cells in their glomeruli, the numbers of such cells increased markedly, in a time-dependent manner, from 2 wk to 24 wk after bone marrow transplantation. Immunohistochemical analyses revealed that most GFP-positive cells in the glomeruli were neither macrophages nor T cells. With the use of a laser-scanning confocal microscope, GFP-positive cells were observed within the mesangium of [GFP-->B6] mice. Furthermore, indirect immunofluorescence assays demonstrated that desmin-positive cells in the glomeruli of these chimeric mice were also positive for GFP. Among glomerular cells isolated from [GFP-->B6] mice 24 wk after bone marrow transplantation and then cultured, the majority of cells (approximately 84%) stained for desmin and approximately 60% of the desmin-positive cells expressed GFP. In addition, these GFP-positive cells in the cultures contracted in response to angiotensinII stimulation. These results suggest that bone marrow-derived cells may have the potential to differentiate into glomerular mesangial cells.

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Documento generato il 10/07/20 alle ore 13:45:02