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Titolo:
Isolation of an androgen-inducible novel lipocalin gene, Arg1, from androgen-dependent mouse mammary Shionogi carcinoma cells
Autore:
Kobayashi, M; Kinouchi, T; Hakamata, Y; Kamiakito, T; Kuriki, K; Suzuki, K; Tokue, A; Fukayama, M; Tanaka, A;
Indirizzi:
Jichi Med Sch, Dept Pathol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Urol, Minami Kawachi, Tochigi 3290498, Japan Jichi MedSch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Biochem, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Ctr Mol Med, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Univ Tokyo, Dept Pathol, Grad Sch Med, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sch Med, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 77, anno: 2001,
pagine: 109 - 115
SICI:
0960-0760(200105)77:2-3<109:IOAANL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-INDUCED EXPRESSION; HUMAN BREAST-CANCER; BETA-CATENIN; APOLIPOPROTEIN-D; PROSTATE-CANCER; CATHEPSIN-B; GROWTH; PROGRESSION; ACTIVATION; PROTEIN;
Keywords:
androgen; lipocalin; cathepsin; PCR-based subtraction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, A Jichi Med Sch, Dept Pathol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 90498, Japan
Citazione:
M. Kobayashi et al., "Isolation of an androgen-inducible novel lipocalin gene, Arg1, from androgen-dependent mouse mammary Shionogi carcinoma cells", J STEROID B, 77(2-3), 2001, pp. 109-115

Abstract

Here we report isolation of an androgen-regulated novel gene from an androgen-dependent mouse mammary Shionogi carcinoma SC-3 cell line. Using a polymerase chain reaction-based subtraction method and Northern blotting analysis, we isolated four androgen-inducible genes from SC-3 cells. Nucleotide sequencings identified three of the genes as cyclin D1, beta -catenin, and fatty acid synthase, respectively, but the fourth, a gene tentatively named as Arg1 (androgen-regulated gene 1), remained undefined. The cloned 2.0-kb sized Arg1 cDNA encoded 414 amino acid sequences. The deduced amino acid sequences, sharing about 30% homology with cathepsin family members at a protein level, had relatively conserved residues around the three proteinase active sites reported earlier. In Northern blotting, Arg1 mRNA was found in kidney. heart, lung, and to a lesser degree, in spleen and liver. Its transcripts were also detected in male reproductive organs on RT-PCR. In addition, its expression levels in prostate were markedly reduced after castration. Unexpectedly, Arg1-expressing COS1 cells showed no significant proteinase activity to various synthesized substrates under neutral or acidic conditions in this study. This might have been due to the replacement of the cysteinyl active site for proteinase to serine residue in the Arg1 amino acid sequences. Given that Arg1 also contains a lipocaline signature known as a binding motif for small hydrophobic molecules at the center of its amino acid sequences, Arg1 is a lipocalin family gene regulated by androgens in prostate and Shionogi carcinoma cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 03/07/20 alle ore 22:55:17