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Titolo:
Cortical fibroblast culture from human biopsies
Autore:
Strutz, F; Renziehausen, A; Dietrich, M; Amin, J; Becker, V; Heeg, M; Rastaldi, MP; Muller, GA;
Indirizzi:
Univ Gottingen, Dept Nephrol & Rheumatol, D-37075 Gottingen, Germany Univ Gottingen Gottingen Germany D-37075 tol, D-37075 Gottingen, Germany San Carlo Borromeo Hosp, Div Nephrol, Milan, Italy San Carlo Borromeo Hosp Milan Italy meo Hosp, Div Nephrol, Milan, Italy
Titolo Testata:
JOURNAL OF NEPHROLOGY
fascicolo: 3, volume: 14, anno: 2001,
pagine: 190 - 197
SICI:
1121-8428(200105/06)14:3<190:CFCFHB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN RENAL FIBROBLASTS; TUBULAR EPITHELIAL-CELLS; INTERSTITIAL FIBROSIS; GLOMERULAR-DISEASES; GROWTH; KIDNEYS; MYOFIBROBLASTS; PROLIFERATION; NEPHROPATHY; PROGRESSION;
Keywords:
kidney fibrosis; extracellular matrix; alpha-smooth muscle actin; CD 44; CD 54; CD 90;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Strutz, F Univ Gottingen, Dept Nephrol & Rheumatol, Robert Koch Str 40, D-37075 Gottingen, Germany Univ Gottingen Robert Koch Str 40 Gottingen Germany D-37075 any
Citazione:
F. Strutz et al., "Cortical fibroblast culture from human biopsies", J NEPHROL, 14(3), 2001, pp. 190-197

Abstract

Background: Tubulointerstitial fibrosis is an integral part of progressiverenal disease. Human cortical fibroblasts are believed to be key effector cells in fibrogenesis. Thus, a reliable culture of these cells is necessaryfor studies of their pathophysiology. Methods: Cortical fibroblast culture from routine kidney biopsies were analyzed and the cells were characterized. Indirect immunofluorescence staining was done after the first passage for cytokeratin, vimentin, alpha -smoothmuscle actin, CD 44, CD 54, CD 68, collagen types I, III, and HLA-DR. We then assessed the utility of the putative fibroblast markers CD 90, prolyl-4-hydroxylase (P4H) and F1b in simultaneous stainings of tubular epithelial cells. Results: During the study period, 49 biopsy cores were cultured and cortical fibroblasts could be successfully established in 21 cases (42.9%). Therewas no relation between the success rate of culture and the degree of interstitial fibrosis, but an association was seen with the time of completion of the first passage. There was a negative correlation between the extent of scarring and the percentage of cytokeratin positive cells (r=-0.66, p <0.001). All primary fibroblasts were negative for factor VIII, HLA-DR, CD 68,and cytokeratin. They expressed alpha -smooth muscle actin and collagen types I and III to variable degrees. There was a robust correlation between die percentage of alpha -smooth muscle actin positive cells and interstitialscarring but no such association with collagen type I or type III positivecells. The three putative fibroblast markers did not prove useful in differentiating between tubular epithelial cells; aid fibroblasts. However, since only fibroblasts stained positive for CD 90 and negative for cytokeratin,these two markers may suffice to distinguish fibroblasts from other renal cellular elements. Conclusions: Cortical renal fibroblasts can be easily cultured from kidneybiopsy cores, though the success rate of pure cultures is below 50%. Staining for CD 90 and cytokeratin may suffice for initial characterization of these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:09:51