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Titolo:
INVESTIGATION OF THE POTENTIAL ROLE OF THE GERM-CELL COMPLEMENT IN CONTROL OF THE EXPRESSION OF TRANSFERRIN MESSENGER-RNA IN THE PREPUBERTAL AND ADULT-RAT TESTIS
Autore:
MAGUIRE SM; MILLAR MR; SHARPE RM; GAUGHAN J; SAUNDERS PTK;
Indirizzi:
MRC,REPROD BIOL UNIT,37 CHALMERS ST EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND
Titolo Testata:
Journal of molecular endocrinology
fascicolo: 1, volume: 19, anno: 1997,
pagine: 67 - 77
SICI:
0952-5041(1997)19:1<67:IOTPRO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; STAGE-DEPENDENT EXPRESSION; SERTOLI CELLS; IMMUNOHISTOCHEMICAL LOCALIZATION; PACHYTENE SPERMATOCYTES; TESTICULAR TRANSFERRIN; SPERMATOGENESIS; RNA; TESTOSTERONE; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S.M. Maguire et al., "INVESTIGATION OF THE POTENTIAL ROLE OF THE GERM-CELL COMPLEMENT IN CONTROL OF THE EXPRESSION OF TRANSFERRIN MESSENGER-RNA IN THE PREPUBERTAL AND ADULT-RAT TESTIS", Journal of molecular endocrinology, 19(1), 1997, pp. 67-77

Abstract

Iron is required for the normal development of germ cells during spermatogenesis. Because these cells have no direct access to systemic iron, there exists a shuttle system involving production and secretion ofthe iron-transporting protein transferrin by the Sertoli cells. Previous reports using cultures of immature Sertoli cells exposed to adult germ cells, or in vivo studies involving germ cell-depleted adult rat testes, concluded that production of transferrin by Sertoli cells is modulated by germ cell complement. In the present study we have used insitu hybridisation with cRNA probes directed against the 5' and 3' ends of transferrin mRNA to examine the pattern of expression of transferrin in the immature and adult rat testis. Adult rats were treated with ethane dimethane sulphonate of methoxyacetic acid (MAA) to manipulate their testosterone levels or germ cell complement respectively. Initial findings obtained using the 3' probe showed a decrease in transferrin mRNA associated with round spermatid depletion. However, these data were not confirmed by in situ hybridisation when the 5' probe was used. The specificity of the probes was examined using Northern blottingand the 3' probe was found to hybridise to the germ cell transcript for hemiferrin even under conditions of high stringency. Examination ofimmature and pubertal rat testes by in situ hybridisation using the 5' transferrin-specific probe found that as early as 14 days of age thelevel of expression of transferrin mRNA was clearly different betweentubules, and the mRNA appeared to be expressed in Leydig cells on andafter day 31. In the adult rat testis, maximal expression of transferrin mRNA was found at stages VIII-XIV, calling into question the interpretation of the results of some previous studies showing expression of transferrin mRN4 at all stages of the spermatogenic cycle. This stage-specific pattern of expression was not altered by acute cell depletion using MAA. However, Northern blot analysis showed a statistically significant increase in transferrin mRNA expression at 7 days after MAAtreatment when pachytene spermatocytes were depleted from tubules at all stages of the spermatogenic cycle at which transferrin is normallyexpressed. In conclusion, we found that transferrin mRNA expression was not modulated by round spermatids as has been reported previously but that meiotic germ Cells may influence expression of transferrin at specific stages of the spermatogenic cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:49:13