Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Human papillomavirus-16-E7 oncoprotein enhances the expression of adhesionmolecules in cervical endothelial cells but not in human umbilical vein endothelial cells
Autore:
DAnna, R; Le Buanec, H; Bizzini, B; Burny, A; Giannouli, C; Zagury, JF; Gallo, RC; Zagury, D; DAlessio, P;
Indirizzi:
CHU Necker Enfants Malad, Dept Biol Cellulaire, F-75015 Paris, France CHU Necker Enfants Malad Paris France F-75015 ire, F-75015 Paris, France Univ Maryland, Inst Human Virol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Human Virol, Baltimore, MD 21201 USA Inst Jules Bordet, B-1000 Brussels, Belgium Inst Jules Bordet Brussels Belgium B-1000 rdet, B-1000 Brussels, Belgium Free Univ Brussels, Dept Biol Mol, Chim Biol Lab, B-1640 Rhode St Genese, Belgium Free Univ Brussels Rhode St Genese Belgium B-1640 ode St Genese, Belgium Univ Paris 06, Lab Physiol Cellulaire, Paris, France Univ Paris 06 ParisFrance is 06, Lab Physiol Cellulaire, Paris, France
Titolo Testata:
JOURNAL OF HUMAN VIROLOGY
fascicolo: 2, volume: 4, anno: 2001,
pagine: 85 - 95
SICI:
1090-9508(200103/04)4:2<85:HPOETE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; IMMUNODEFICIENCY-VIRUS TYPE-1; E-SELECTIN; CELLULAR IMMUNOSUPPRESSION; ONCOGENIC PROTEIN; CANCER-CELLS; TUMOR-CELLS; HPV-16 E7; METASTASIS; ANGIOGENESIS;
Keywords:
HPV-16-E7; endothelial cells; adhesion molecules; cervical cancer; TNF-alpha; H2O2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: D'Alessio, P CHU Necker Enfants Malad, Dept Biol Cellulaire, 156 Rue Vaugirard, F-75015Paris, France CHU Necker Enfants Malad 156 Rue Vaugirard Paris France F-75015
Citazione:
R. D'Anna et al., "Human papillomavirus-16-E7 oncoprotein enhances the expression of adhesionmolecules in cervical endothelial cells but not in human umbilical vein endothelial cells", J HUMAN VIR, 4(2), 2001, pp. 85-95

Abstract

Objectives: E7 is one of the oncoproteins encoded by human papillomavirus-16 (HPV-16), the major etiologic factor responsible for cervical cancer. Human papillomavirus-16-E7 expressed by human uterine cen;ix carcinoma cells is also released in the extracellular compartment where it induces immune suppression. We investigated whether E: was also responsible for the enhanced endothelial adhesiveness required in cancer progression. Study Design/Methods: We treated cervical microvascular endothelial cells (CrMVEn) and human umbilical vein endothelial cells (HUVEC) with E7, tumor necrosis factor-alpha (TNF-alpha), and hydrogen peroxide (H2O2) and measured the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1),and vascular cell adhesion molecule-1 (VCAM-1) by fluorescent-activated cell sorter analysis. Results: E7 strongly induced the expression of E-selectin, ICAM-1, and VCAM-1 in CrMVEn, but not in HUVEC. Tumor necrosis factor-a further increased the endothelial expression of adhesion molecules in CrMVEn. Hydrogen peroxide pre treatment resulted in an enhanced ICAM-1 and a decreased E-selectin and VCAM-1 expression. We also show indirect effects when endothelial cellswere stimulated with the supernatant of E7-pretreated macrophages. Conclusions: These results show that HPV-16-E7 oncoprotein strongly induces adhesion molecules expression in organ-specific endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:53:35