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Titolo:
Evaluation of the primary effect of brefeldin A treatment upon herpes simplex virus assembly
Autore:
Dasgupta, A; Wilson, DW;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Dev & Mol Biol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 7
pagine: 1561 - 1567
SICI:
0022-1317(200107)82:<1561:EOTPEO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; ANTEROGRADE TRANSPORT; GENE-PRODUCT; CELL-LINES; UL20 GENE; EGRESS; MATURATION; TYPE-1; DNA; VIRIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Wilson, DW Yeshiva Univ Albert Einstein Coll Med, Dept Dev & Mol Biol, 1300 Morris PkAve, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave Bronx NY USA 10461
Citazione:
A. Dasgupta e D.W. Wilson, "Evaluation of the primary effect of brefeldin A treatment upon herpes simplex virus assembly", J GEN VIROL, 82, 2001, pp. 1561-1567

Abstract

Addition of the drug brefeldin A (BFA) to cells infected by herpes simplexvirus (HSV) type 1 is known to result in a complex pattern of defects in particle assembly. BFA-treated, infected cells accumulate perinuclear enveloped virions and non-enveloped ('naked') cytoplasmic capsids, and it has been difficult to interpret these data in terms of the assembly pathway of HSVand the known effects of BFA on the secretory apparatus. Since BFA is a cytotoxic drug, and earlier studies commonly examined the effects of long-term BFA incubations on infected cells, it was hypothesized that the drug could have pleiotropic and indirect effects on HSV assembly. To test this, use was made of an HSV synchronized assembly assay, in which cells are infectedwith the virus mutant tsProt,A and maintained at 39 degreesC to induce reversible accumulation of a population of procapsids. By first adding BFA andthen shifting these cells to 31 degreesC for 3 h to allow the accumulated procapsids to mature, it was possible to test the effect of short-term BFA treatment on only those HSV assembly events that are downstream of procapsid maturation. Under these conditions, it was found that procapsids matured and packaged the viral genome normally, but remained non-enveloped and failed to exit the nucleus. It is concluded that the primary effect of BFA on HSV replication is to inhibit budding at the inner nuclear membrane.

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Documento generato il 04/12/20 alle ore 12:47:52