Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Essential requirement for c-kit and common gamma chain in thymocyte development cannot be overruled by enforced expression of bcl-2
Autore:
Rodewald, HR; Waskow, C; Haller, C;
Indirizzi:
Univ Ulm, Dept Immunol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081 niv Ulm, Dept Immunol, D-89081 Ulm, Germany Basel Inst Immunol, CH-4005 Basel, Switzerland Basel Inst Immunol Basel Switzerland CH-4005 CH-4005 Basel, Switzerland
Titolo Testata:
JOURNAL OF EXPERIMENTAL MEDICINE
fascicolo: 12, volume: 193, anno: 2001,
pagine: 1431 - 1437
SICI:
0022-1007(20010618)193:12<1431:ERFCAC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL DEVELOPMENT; RECEPTOR-DEFICIENT MICE; CYTOKINE RECEPTORS; MUTANT MICE; INTERLEUKIN-7; LYMPHOPOIESIS; MATURATION; APOPTOSIS; EXPANSION; THYMUS;
Keywords:
growth factors; c-kit; common cytokine receptor gamma chain; bcl-2; T cell development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Rodewald, HR Univ Ulm, Dept Immunol, Albert Einstein Allee 11, D-89081 Ulm, Germany Univ Ulm Albert Einstein Allee 11 Ulm Germany D-89081 ermany
Citazione:
H.R. Rodewald et al., "Essential requirement for c-kit and common gamma chain in thymocyte development cannot be overruled by enforced expression of bcl-2", J EXP MED, 193(12), 2001, pp. 1431-1437

Abstract

The thymus in mice lacking both the receptor tyrosine kinase c-kit and thecommon cytokine receptor gamma chain (gamma (c)) is alymphoid because these receptors provide essential signals at the earliest stages of thymocyte development. The signals transduced by these receptors potentially regulate proliferation, survival, or differentiation, but the contribution of each receptor to distinct intracellular signaling cascades is only poorly defined. Here, we have examined whether en forced expression of Bcl-2 can rescue thymocyte development in c-kit and y, single or double mutant mice. A bcl-2 transgene (E mu -bcl-2-25; expressed in the T cell lineage) was introduced into (a) c-kit and gamma (c) wild-type (c-kit(+)gamma (+)(c)bcl(+)), (b) c-kit-deficient (c-kit(-)gamma (+)(c)bcl(+)), (c) gamma (c)-deficient (c-kit()gamma (-)(c)bcl(+)), or (d) c-kit and gamma (c) double-deficient mice (c-kit(-)gamma (-)(c)bcl(+)). The bcl-2 transgene was functionally active in wild-type and c-kit or gamma (c) single mutants, as it promoted survival of ex vive isolated thymocytes, including pro-T cells. In vive, however, transgenic Bcl-2 did not release T cell precursors from their phenotypic block and failed to increase progenitor or total thymocyte cellularity in c-kit orgamma (c) single or double mutants. These data argue strongly against a role for Eel-2 as a key mediator in signaling pathways linked to cytokine andgrowth factor receptors driving early thymocyte development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:04:03