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Titolo:
Anti-JL1 antibody-conjugated poly (L-lysine) for targeted gene delivery toleukemia T cells
Autore:
Suh, WH; Chung, JK; Park, SH; Kim, SW;
Indirizzi:
Univ Utah, Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Chem, Salt Lake City, UT 84112 USA Seoul Natl Univ, Dept Nucl Med, Seoul, South Korea Seoul Natl Univ SeoulSouth Korea iv, Dept Nucl Med, Seoul, South Korea Seoul Natl Univ Hosp, Dept Pathol, Seoul 110744, South Korea Seoul Natl Univ Hosp Seoul South Korea 110744 Seoul 110744, South Korea
Titolo Testata:
JOURNAL OF CONTROLLED RELEASE
fascicolo: 1-3, volume: 72, anno: 2001,
pagine: 171 - 178
SICI:
0168-3659(20010514)72:1-3<171:AAP(FT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA; CARRIER; SYSTEM; JL1;
Keywords:
gene delivery; plasmid; leukemia; Molt 4 cell; anti-JL1 antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SW Univ Utah, Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, BPRB Rm 205, Salt Lake City, UT 84112 USA Univ Utah BPRB Rm 205 Salt Lake City UT USA 84112 ty, UT 84112 USA
Citazione:
W.H. Suh et al., "Anti-JL1 antibody-conjugated poly (L-lysine) for targeted gene delivery toleukemia T cells", J CONTR REL, 72(1-3), 2001, pp. 171-178

Abstract

We have designed the gene delivery carrier targeted to Molt 4 cells, humanleukemia T cells, using monoclonal antibody against leukemia-specific JL1 antigen, anti-JL1 antibody, as a targeting moiety. Anti-JL1 antibody has been proven to bind to JL1 antigen and subsequently be internalized into Molt4 cells, demonstrating that anti-JL1 antibody has the potential as a targeting ligand for leukemia-specific gene transfer. Anti-JL1 antibody was modified with the heterobifunctional crosslinker, PDPH, at carbohydrate sites and conjugated to thiolated poly-L-lysine (PLL) via disulfide bridges. The composition and antigen binding affinity of antibody-PLL conjugates were analyzed by the amino acid analysis and the flow cytometry, respectively. Antibody-PLL conjugates neutralized pSV-beta -galactosidase plasmid DNA at 5:1 weight ratio and condensed into about 200-300-nm complexes. DNA/antibody-PLL complexes were effectively internalized into Molt 4 cells after 4 h incubation at 37 degreesC and showed significantly higher in vitro transfection efficiency than DNA/PLL complexes and DNA/Lipofectin(TM) formulation due tothe targeting effect of receptor-mediated endocytosis induced by anti-JL1 antibody. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:01:00