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Titolo:
Effect of temperature on serum protein binding characteristics of phenytoin in monotherapy paediatric patients with epilepsy
Autore:
Kodama, H; Kodama, Y; Itokazu, N; Shinozawa, S; Kanemaru, R; Sugimoto, T;
Indirizzi:
Miyazaki Med Coll, Dept Pharm, Kiyotake, Miyazaki 8891692, Japan Miyazaki Med Coll Kiyotake Miyazaki Japan 8891692 Miyazaki 8891692, Japan Miyazaki Med Coll, Dept Paediat, Kiyotake, Miyazaki 8891692, Japan Miyazaki Med Coll Kiyotake Miyazaki Japan 8891692 Miyazaki 8891692, Japan Jichi Med Sch, Dept Clin Pharmacol, Minami Kawachi, Tochigi, Japan Jichi Med Sch Minami Kawachi Tochigi Japan inami Kawachi, Tochigi, Japan Kanemaru Neurosurg Hosp, Miyazaki, Japan Kanemaru Neurosurg Hosp Miyazaki Japan Neurosurg Hosp, Miyazaki, Japan
Titolo Testata:
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS
fascicolo: 3, volume: 26, anno: 2001,
pagine: 175 - 179
SICI:
0269-4727(200106)26:3<175:EOTOSP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VALPROIC ACID; PLASMA; DIPHENYLHYDANTOIN; PARAMETERS;
Keywords:
epilepsy; monotherapy; paediatrics; phenytoin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Kodama, H Miyazaki Med Coll, Dept Pharm, Kiyotake, Miyazaki 8891692, JapanMiyazaki Med Coll Kiyotake Miyazaki Japan 8891692 891692, Japan
Citazione:
H. Kodama et al., "Effect of temperature on serum protein binding characteristics of phenytoin in monotherapy paediatric patients with epilepsy", J CLIN PH T, 26(3), 2001, pp. 175-179

Abstract

Objectives: To determine the effects of temperature on binding characteristics of phenytoin to serum proteins in paediatric patients with epilepsy. Method: Serum samples examined in the study were obtained from 41 paediatric patients (23 male, 18 female) with epilepsy on phenytoin monotherapy. Their age ranged from 1 to 15 years (mean +/- SD, 10.2 +/- 4.0 years). Protein binding of phenytoin was evaluated by ultrafiltration under current laboratory routine conditions (25 +/- 3 degreesC) or at a temperature of 37 degreesC. The in vivo binding parameters of phenytoin to serum proteins were determined using a binding equation derived from the Scatchard equation for aone-site binding model. Results: Significant differences were observed in serum concentrations of unbound phenytoin at the two temperatures (P < 0.05). The mean association constant (K) of phenytoin to serum proteins is 0.016 L/<mu>mol at 25 +/- 3 degreesC and 0.009 L/mu mol at 37 degreesC, while mean total concentration of binding sites (n(Pt)) seems to be similar between the two temperatures (682 mu mol/L for 25 +/- 3 degreesC and 746 mu mol/L for 37 degreesC). Significant differences were observed in binding characteristics of phenytoin toserum proteins for the different temperature conditions of ultrafiltration(P < 0.05). Conclusion: Our study confirms that binding affinity for phenytoin-serum protein interaction is approximately 44% lower at 37 <degrees>C than at 25 +/- 3 degreesC and consequently, binding potential (K.n(Pt)) is approximately 38% lower at 37 degreesC than at 25 +/- 3 degreesC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:14:59