Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The polymorphism in the caudal-related homeodomain protein Cdx-2 binding element in the human vitamin D receptor gene
Autore:
Arai, H; Miyamoto, KI; Yoshida, M; Yamamoto, H; Taketani, Y; Morita, K; Kubota, M; Yoshida, S; Ikeda, M; Watabe, F; Kanemasa, Y; Takeda, E;
Indirizzi:
Univ Tokushima, Sch Med, Dept Clin Nutr, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 in Nutr, Tokushima 7708503, Japan Univ Tokushima, Sch Med, Dept Nutr Sci, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 utr Sci, Tokushima 7708503, Japan Okayama Prefectural Univ, Fac Hlth & Welf Sci, Dept Nutr, Okayama, Japan Okayama Prefectural Univ Okayama Japan f Sci, Dept Nutr, Okayama, Japan Okayama Prefectural Univ, Fac Hlth & Welf Sci, Dept Nursing, Okayama, Japan Okayama Prefectural Univ Okayama Japan ci, Dept Nursing, Okayama, Japan Tamano Inst Hlth & Human Serv, Okayama, Japan Tamano Inst Hlth & Human Serv Okayama Japan Human Serv, Okayama, Japan
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 7, volume: 16, anno: 2001,
pagine: 1256 - 1264
SICI:
0884-0431(200107)16:7<1256:TPITCH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MINERAL DENSITY; INTESTINE-SPECIFIC TRANSCRIPTION; START CODON POLYMORPHISM; CALCIUM-ABSORPTION; JAPANESE WOMEN; PROXIMAL FEMUR; RAT; EXPRESSION; AGE; ALLELES;
Keywords:
vitamin D receptor; gene promoter; caudal-related homeodomain protein; intestine-specific transcription; calcium absorption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Arai, H Univ Tokushima, Sch Med, Dept Clin Nutr, Kuramoto Cho 3, Tokushima7708503, Japan Univ Tokushima Kuramoto Cho 3 Tokushima Japan 7708503 8503, Japan
Citazione:
H. Arai et al., "The polymorphism in the caudal-related homeodomain protein Cdx-2 binding element in the human vitamin D receptor gene", J BONE MIN, 16(7), 2001, pp. 1256-1264

Abstract

The major physiological activity of 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3] is the regulation of calcium absorption in the small intestine, and the level of vitamin D receptor (VDR) is an important factor in this regulation. In a previous study, we indicated that the caudal-related homeodomain Cdx-2 played an important role in the intestine-specific transcription of the human VDR gene, In this study, the polymorphism was identified in the core sequence 5 ' -ATAAAAACTTAT-3 ' in the Cdx-2 binding site in the VDR gene promoter. In 261 Japanese women with genotyped VDR polymorphisms, 48 were genotype Cdx-A (adenine at -3731 nucleotides [nt] relative to the transcription start site of human VDR gene 5-ATAABBACTTAT), 82 were genotype Cdx-G (guanine at -3731 nt, 5 ' -GTAAAAACTTAT-3 '), and 131 were genotype Cdx-A/G (heterozygote). In postmenopausal Japanese women, the bone mineral density (BMD) in the lumbar spine (L2-L4) with the Cdx-G homozygote was 12% lower than that with the Cdx-A homozygote (p < 0.05), In electrophoretic gel mobility shift assay (ER;ISA), the oligonucleotide with Cdx-G allele markedly decreased the binding to Cdx-2 compared with that in the Cdx-A allele, The transcriptional activity of the VDR promoter with Cdx-G allele was decreased to 70% of the Cdx-A allele, In addition, in the herpes simplex virus thymidine kinase promoter, the Cdx-2 binding element with the G allele showed significantly lower transcriptional activity than that of the A allele. Thus, the polymorphism in the Cdx-2 binding site of the VDR gene (Cdx-polymorphism) would affect the expression of VDR in the small intestine. In addition, this polymorphism may modulate BMD in postmenopausal Japanese women.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 23:34:22