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Titolo:
Application of the PHO5-gene-fusion technology to molecular genetics and biotechnology in yeast
Autore:
Harashima, S; Kaneko, Y;
Indirizzi:
Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 technol, Suita, Osaka 5650871, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 4, volume: 91, anno: 2001,
pagine: 325 - 338
SICI:
1389-1723(200104)91:4<325:AOTPTT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROMOTER-PROBE VECTOR; CELL TYPE CONTROL; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ACTIVATOR; BETA-GALACTOSIDASE; ACID-PHOSPHATASE; ESCHERICHIA-COLI; REPORTER GENE; NUCLEOTIDE-SEQUENCE; CANDIDA-ALBICANS;
Keywords:
PHO5 fusion; reporter gene; promoter analysis; trans-acting mutation; DNA binding protein; one-hybrid system; molecular breeding; industrial strains;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
97
Recensione:
Indirizzi per estratti:
Indirizzo: Harashima, S Osaka Univ, Grad Sch Engn, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-1 Yamadaoka Suita Osaka Japan 5650871 871, Japan
Citazione:
S. Harashima e Y. Kaneko, "Application of the PHO5-gene-fusion technology to molecular genetics and biotechnology in yeast", J BIOSCI BI, 91(4), 2001, pp. 325-338

Abstract

Modern biological scientists employ numerous approaches for solving their problems. Among these approaches, the gene fusion is surely one of the well-established valuable tools in various fields of biological sciences. A wide range of applications have been developed to analyze a variety of biological phenomena such as transcriptional regulation, pre-mRNA processing, mRNAdecay, translation, protein localization and even protein transport in both prokaryotic and eukaryotic organisms. Gene fusions were also used for thestudy of protein purification, protein structure, protein folding, protein-protein interaction and protein-DNA interaction. Here, we describe applications of gene fusion technology using the Saccharomyces cerevisiae PHO5 gene encoding repressible acid phosphatase to molecular genetics and biotechnology in S. cerevisiae. Using the PHO5 gene fusion as a reporter, we have identified several cis- and trans-acting genes of S. cerevisiae which are involved in splicing of pre-mRNA, biosynthesis of amino acids, ubiquitin-dependent protein degradation, signal transduction of oxygen and unsaturated fatty acid, regulation of transcription by the nucleosome and chromatin. The PHO5 gene fusions exhibiting the mating-type specific expression were also generated to develop a breeding technique for industrial yeast. It is concluded that the PHO5 gene fusion is extremely useful and should be further exploited to investigate various cellular steps of the eukaryotic gene expression.

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Documento generato il 09/04/20 alle ore 07:38:26