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Titolo:
Calcium transients in 1B5 myotubes lacking ryanodine receptors are relatedto inositol trisphosphate receptors
Autore:
Estrada, M; Cardenas, C; Liberona, JL; Carrasco, MA; Mignery, GA; Allen, PD; Jaimovich, E;
Indirizzi:
Univ Chile, Fac Med, Inst Ciencias Biomed, Santiago 70005, Chile Univ Chile Santiago Chile 70005 t Ciencias Biomed, Santiago 70005, Chile Loyola Univ, Dept Physiol, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 niv, Dept Physiol, Maywood, IL 60153 USA Brigham & Womens Hosp, Dept Anesthesia, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 nesthesia, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 25, volume: 276, anno: 2001,
pagine: 22868 - 22874
SICI:
0021-9258(20010622)276:25<22868:CTI1ML>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+ RELEASE CHANNELS; MYOGENIC CELL-LINE; SKELETAL-MUSCLE; 1,4,5-TRISPHOSPHATE RECEPTOR; GENE-EXPRESSION; NUCLEUS; UNITS; MEMBRANES; PATHWAYS; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Jaimovich, E Casilla 70005, Santiago 6530499, Chile Casilla 70005 Santiago Chile 6530499 antiago 6530499, Chile
Citazione:
M. Estrada et al., "Calcium transients in 1B5 myotubes lacking ryanodine receptors are relatedto inositol trisphosphate receptors", J BIOL CHEM, 276(25), 2001, pp. 22868-22874

Abstract

Potassium depolarization of skeletal myotubes evokes slow calcium waves that are unrelated to contraction and involve the cell nucleus (Jaimovich, E., Reyes, R., Liberona, J. L., and Powell, J. A. (2000) Am. J. Physiol. 278,C998-C1010). Studies were done in both the 1B5 (Ry53-/-) murine "dyspedic"myoblast cell line, which does not express any ryanodine receptor isoforms(Moore, R. A, Nguyen, H., Galceran, J., Pessah, I. N., and Alien, P. D. (1998) J. Cell Biol. 140, 843-851), and C2C12 cells, a myoblast cell line that expresses all three isoforms. Although 1B5 cells lack ryanodine binding, they bind tritiated inositol (1,4,5)-trisphosphate. Both type 1 and type 3 inositol trisphosphate receptors were immune-located in the nuclei of both cell types and were visualized by Western blot analysis. After stimulation with 47 mM K+, inositol trisphosphate mass raised transiently in both cell types. Both fast calcium increase and slow propagated calcium signals were seen in C2C12 myotubes. However, 1B5 myotubes (as well as ryanodine-treatedC2C12 myotubes) displayed only a long-lasting, non-propagating calcium increase, particularly evident in the nuclei. Calcium signals in 1B5 myotubes were almost completely blocked by inhibitors of the inositol trisphosphate pathway: U73122, 2-aminoethoxydiphenyl berate, or xestospongin C. Results support the hypothesis that inositol trisphosphate mediates slow calcium signals in muscle cell ryanodine receptors, having a role in their time courseand propagation.

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Documento generato il 15/07/20 alle ore 07:47:53