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Titolo:
A recycling pathway for resecretion of internalized apolipoprotein E in liver cells
Autore:
Swift, LL; Farkas, MH; Major, AS; Valyi-Nagy, K; Linton, MF; Fazio, S;
Indirizzi:
Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 pt Pathol, Nashville, TN 37232 USA Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Dept Med, Nashville, TN 37232 USA Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Pharmacol, Nashville, TN 37232 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 25, volume: 276, anno: 2001,
pagine: 22965 - 22970
SICI:
0021-9258(20010622)276:25<22965:ARPFRO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE PERITONEAL-MACROPHAGES; BONE-MARROW TRANSPLANTATION; CHOLESTEROL EFFLUX; RECEPTOR-BINDING; TRANSGENIC MICE; DEFICIENT MICE; HEPATIC-UPTAKE; E EXPRESSION; APOPROTEIN-E; BETA-VLDL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Swift, LL Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Nashville, TN 37232 USA
Citazione:
L.L. Swift et al., "A recycling pathway for resecretion of internalized apolipoprotein E in liver cells", J BIOL CHEM, 276(25), 2001, pp. 22965-22970

Abstract

We have investigated the recycling of apoE in livers of apoE(-/-) mice transplanted with wild type bone marrow (apoE(+/+) --> apoE(-/-)), a model in which circulating apoE is derived exclusively from macrophages. Nascent Gels lipoproteins were recovered from livers of apoE(+/+) --> apoE(-/-) mice 8weeks after transplantation. ApoE was identified with nascent d < 1.006 and with d 1.006-1.210 g/ml lipoproteins at a level similar to6% that of nascent lipoproteins from C57BL/6 mice. Hepatocytes from apoE(+/+) --> apoE(-/-) mice were isolated and cultured in media free of exogenous apoE, ApoE wasfound in the media primarily on the d < 1.006 g/ml fraction, indicating a resecretion of internalized apoprotein. Secretion of apoE from C57BL/6 hepatocytes was consistent with constitutive production, whereas the majority of apoE secreted from apoE(+/+) --> apoE(-/-) hepatocytes was recovered in the last 24 h of culture, This suggests that release may be triggered by accumulation of an acceptor, such as very low density lipoproteins, in the media. In agreement with the in vivo data, total recovery of apoE from apoE(+/) --> apoE(-/-) hepatocytes was similar to6% that of the apoE recovered from C57BL/6 hepatocytes. Since plasma apoE levels in the transplanted mice are similar to 10% of control levels, the findings indicate that up to 60% of the internalized apoE may be reutilized under physiologic conditions. These studies provide definitive evidence for the sparing of apoE and its routing through the secretory pathway and demonstrate that internalized apoE can be resecreted in a quantitatively significant fashion.

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Documento generato il 27/11/20 alle ore 15:33:40