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Titolo:
Mechanisms by which intracellular calcium induces susceptibility to secretory phospholipase A(2) in human erythrocytes
Autore:
Smith, SK; Farnbach, AR; Harris, FM; Hawes, AC; Jackson, LR; Judd, AM; Vest, RS; Sanchez, S; Bell, JD;
Indirizzi:
Brigham Young Univ, Dept Zool, Provo, UT 84602 USA Brigham Young Univ Provo UT USA 84602 niv, Dept Zool, Provo, UT 84602 USA Univ Illinois, Lab Flourescence Dynam, Urbana, IL 61801 USA Univ IllinoisUrbana IL USA 61801 lourescence Dynam, Urbana, IL 61801 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 25, volume: 276, anno: 2001,
pagine: 22732 - 22741
SICI:
0021-9258(20010622)276:25<22732:MBWICI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-CELL DEFORMABILITY; PALMITIC ACID; RED-CELLS; GENERALIZED POLARIZATION; SIGNAL-TRANSDUCTION; FLUORESCENT-PROBE; MEMBRANE; ASYMMETRY; VESICLES; DIPALMITOYLPHOSPHATIDYLCHOLINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Bell, JD Brigham Young Univ, Dept Zool, Provo, UT 84602 USA Brigham Young Univ Provo UT USA 84602 Zool, Provo, UT 84602 USA
Citazione:
S.K. Smith et al., "Mechanisms by which intracellular calcium induces susceptibility to secretory phospholipase A(2) in human erythrocytes", J BIOL CHEM, 276(25), 2001, pp. 22732-22741

Abstract

Exposure of human erythrocytes to the calcium ionophore ionomycin renderedthem susceptible to the action of secretory phospholipase A(2) (sPLA(2)). Analysis of erythrocyte phospholipid metabolism by thin-layer chromatography revealed significant hydrolysis of both phosphatidylcholine and phosphatidylethanolamine during incubation with ionomycin and sPLA(2). Several possible mechanisms for the effect of ionomycin were considered. Involvement of intracellular phospholipases A, was excluded since inhibitors of these enzymes had no effect. Assessment of membrane oxidation by cis-parinaric acid fluorescence and comparison to the oxidants diamide and phenylhydrazine revealed that oxidation does not participate in the effect of iono- mycin. Incubation with ionomycin caused classical physical changes to the erythrocyte membrane such as morphological alterations (spherocytosis), translocation of aminophospholipids to the outer leaflet of the membrane, and release of microvesicles. Experiments with phenylhydrazine, KCI, quinine, merocyanine 540, the calpain inhibitor E-64d, and the scramblase inhibitor R5421 revealed that neither phospholipid translocation nor vesicle release was required to induce susceptibility. Results from fluorescence spectroscopy and two-photon excitation scanning microscopy using the membrane probe laurdan arguedthat susceptibility to sPLA(2) is a consequence of increased order of membrane lipids.

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Documento generato il 16/07/20 alle ore 05:49:06