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Titolo:
Identification of a simian immunodeficiency virus reverse transcriptase variant with enhanced replicational fidelity in the late stage of viral infection
Autore:
Diamond, TL; Kimata, J; Kim, B;
Indirizzi:
Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14672 USAUniv Rochester Rochester NY USA 14672 & Immunol, Rochester, NY 14672 USA SW Fdn Biomed Res, Dept Virol & Immunol, San Antonio, TX 78245 USA SW Fdn Biomed Res San Antonio TX USA 78245 nol, San Antonio, TX 78245 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 26, volume: 276, anno: 2001,
pagine: 23624 - 23631
SICI:
0021-9258(20010629)276:26<23624:IOASIV>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYNAMICS IN-VIVO; GENETIC-VARIATION; ERROR SPECIFICITY; ESCHERICHIA-COLI; DRUG-RESISTANCE; DNA-SYNTHESIS; MUTATION-RATE; TYPE-1; HIV-1; POLYMERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, B Univ Rochester, Med Ctr, Dept Microbiol & Immunol, 601 Elmwood Ave,Box 672, Rochester, NY 14672 USA Univ Rochester 601 Elmwood Ave,Box 672 Rochester NY USA 14672 2 USA
Citazione:
T.L. Diamond et al., "Identification of a simian immunodeficiency virus reverse transcriptase variant with enhanced replicational fidelity in the late stage of viral infection", J BIOL CHEM, 276(26), 2001, pp. 23624-23631

Abstract

Genomic hypermutation of human and simian immunodeficiency viruses (HIV and SIV) enables these viruses to adapt and escape from various types of antiviral selection by altering the molecular properties of viral gene products. In this study, we examined whether the biochemical and catalytic properties of SIV DNA polymerases (reverse transcriptases; RT) can change during the course of viral infection. For this test, we analyzed RTs obtained from two SIV clones, SIVMNE CL8 and SIVMNE 170. SIVMNE 170 was isolated during the late symptomatic phase of infection with the parental strain, SIVMNE CL8,We found these two RTs have identical DNA polymerase specific activities and kinetics with three different DNA and RNA templates. In addition, the processivity of these two SIV RT proteins mere also similar. However, as demonstrated by a misincorporation assay, the SIVMNE 170 RT showed much higher fidelity than SIVMNE CL8, The fidelity difference between these two SIV RTswas also confirmed by a steady state kinetic fidelity assay. These findings suggest that the fidelity of lentiviral RTs may change during the course of viral infection, possibly in response to alterations of host anti-viral immune capability. In addition, our sequence analysis of these two RT genesproposes possible structural strategies that the virus may employ to alterRT fidelity.

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Documento generato il 01/10/20 alle ore 04:06:59