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Titolo:
Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2
Autore:
de Pedro, MA; Donachie, WD; Holtje, JV; Schwarz, H;
Indirizzi:
Univ Autonoma Madrid, Fac Ciencias, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 o Ochoa, E-28049 Madrid, Spain Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 3JR Midlothian, Scotland Max Planck Inst Entwicklungsbiol, D-7400 Tubingen, Germany Max Planck InstEntwicklungsbiol Tubingen Germany D-7400 bingen, Germany
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 14, volume: 183, anno: 2001,
pagine: 4115 - 4126
SICI:
0021-9193(200107)183:14<4115:CSMSIE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SHAPE GENES; PEPTIDOGLYCAN SYNTHESIS; CHEMICAL-COMPOSITION; DIVISION PLANES; FTSZ RING; ELONGATION; MECILLINAM; MUTANTS; GROWTH; WALL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: de Pedro, MA Univ Autonoma Madrid, Fac Ciencias, CSIC, Ctr Biol Mol SeveroOchoa, Campus Cantoblanco, E-28049 Madrid, Spain Univ Autonoma Madrid Campus Cantoblanco Madrid Spain E-28049
Citazione:
M.A. de Pedro et al., "Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2", J BACT, 183(14), 2001, pp. 4115-4126

Abstract

The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Holtje, and H. Schwarz, J. Bacteriol. 179:2823-2834, 1997). Inactivation of these proteins either by amdinocillin treatment or by mutations in the corresponding genes, pbpA and rodA, respectively, leads to the generation of round, osmotically stable cells. In normal rod-shaped cells, new murein precursors are incorporated all over the lateral wall in a diffuse manner, being mixed up homogeneously with preexisting material, except during septation, when strictly localized murein synthesis occurs. In contrast, in rounded cells, incorporation of new precursors is apparently a zonal process, localized at positions at which division had previously taken place. Consequently, there is no mixing of new and old murein. Old murein is preserved for long periods of timein large, well-defined areas. We propose that the observed patterns are the result of a failure to switch off septal murein synthesis at the end of septation events. Furthermore, the segregation results confirm that round cells of rodA mutants do divide in alternate, perpendicular planes as previously proposed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:53:19