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Titolo:
Effects of sulfur mustard on the basal cell adhesion complex
Autore:
Werrlein, RJ; Madren-Whalley, JS;
Indirizzi:
USA, Med Res Inst Chem Def, Aberdeen Proving Ground, MD 21010 USA USA Aberdeen Proving Ground MD USA 21010 een Proving Ground, MD 21010 USA
Titolo Testata:
JOURNAL OF APPLIED TOXICOLOGY
, volume: 20, anno: 2000, supplemento:, 1
pagine: S115 -
SICI:
0260-437X(200012)20:<S115:EOSMOT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT PROTEINS; EPIDERMOLYSIS-BULLOSA SIMPLEX; STRATIFIED SQUAMOUS EPITHELIA; COILED-COIL; ALPHA(6)BETA(4) INTEGRIN; CYTOPLASMIC DOMAIN; GENETIC-DISORDERS; EPIDERMAL KERATIN; CULTURED-CELLS; CDNA SEQUENCE;
Keywords:
sulfur mustard; basal cell; keratins; K5; K14; integrins; alpha(6); beta(4);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Werrlein, RJ USA, Med Res Inst Chem Def, Aberdeen Proving Ground, MD 21010USA USA Aberdeen Proving Ground MD USA 21010 round, MD 21010 USA
Citazione:
R.J. Werrlein e J.S. Madren-Whalley, "Effects of sulfur mustard on the basal cell adhesion complex", J APPL TOX, 20, 2000, pp. S115

Abstract

Among the most intriguing questions about sulfur mustard (di(2-chloroethyl) sulfide) is why basal cells are the primary targets of its vesicating lesions, To investigate this problem, replicate cultures of human epidermal keratinocytes (HEK) were grown from normal skin and exposed to 400 muM sulfurmustard (HD) for 5 min. Using fluorescein isothiocyanate (FITC)-conjugatedantibodies, confocal laser microscopy and image analyses, we found that inearly passages, sham-treated HEK maintained in a 0.15 mM Ca2+ medium continued to express keratins K5 and K14 as well as alpha (6)beta (4)-integrin. Both K5 and K14 are intermediate filaments characteristic of basal cells and linked with attachment mechanisms effecting epidermolysis bullosa simplex, a family of blistering skin diseases. Acute exposure to HD caused a statistically significant (P < 0.01) 30.74% decrease in K14 fluorescence within Ih of exposure. Within 2 h of exposure, K14 fluorescence decreased to near-zero values. The loss in expression of K14 was progressive and occurred well before the expected appearance of in vivo blisters, which have a dose-dependent, clinical latent phase of 8-24 h, Acute exposure to HD also caused astatistically significant (P<0.002) decrease in expression of beta (4) an integrin which has been associated with junctional epidermolysis bullosa (JEB), Disruption of K14 and alpha (6)beta (4)-integrin may be early events in the HD injury pathway; however, they had no immediate or obvious effect on cell to substrate attachment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:04:59