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Titolo:
Microvascular and interstitial Po-2 measurements in rat skeletal muscle byphosphorescence quenching
Autore:
Shibata, M; Ichioka, S; Ando, J; Kamiya, A;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Biomed Engn, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 med Engn, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 1, volume: 91, anno: 2001,
pagine: 321 - 327
SICI:
8750-7587(200107)91:1<321:MAIPMI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER STRIATED-MUSCLE; OXYGEN-TRANSPORT; HETEROGENEOUS SYSTEMS; PO2 MEASUREMENTS; TISSUE; TENSION; ARTERIOLES; MICROCIRCULATION; DISTRIBUTIONS; GRADIENTS;
Keywords:
oxygen transport; intravital microscope; microcirculation; palladium-porphyrin; cremaster muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Shibata, M Univ Tokyo, Grad Sch Med, Dept Biomed Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 okyo 1130033, Japan
Citazione:
M. Shibata et al., "Microvascular and interstitial Po-2 measurements in rat skeletal muscle byphosphorescence quenching", J APP PHYSL, 91(1), 2001, pp. 321-327

Abstract

To clarify the transport of O-2 across the microvessels in skeletal muscle, we designed an intravital laser microscope that utilizes a phosphorescence quenching technique to determine both the microvascular and tissue PO2. After we injected the phosphorescent probe into systemic blood, phosphorescence excited by a N-2-dye pulse laser was detected with a photomultiplier over a 10 mm in diameter area. In vitro and in vivo calibrations confirmed that the present method is accurate for PO2 measurements in the range of 7-90Torr (r = 0.958) and has a rapid response time. This method was then used to measure the PO2 of microvessels with different diameters (40-130 mum) and of interstitial spaces in rat cremaster muscle. These measurements showeda significant drop in PO2 in the arterioles after branching (from 74.6 to 46.6 Torr) and the presence of a large PO2 gradient at the blood-tissue interface of arterioles (15-20 Torr). These findings suggest that capillaries are not the sole source of oxygen supply to surrounding tissue.

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Documento generato il 01/12/20 alle ore 07:42:47