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Titolo:
Zinc inhibition of group I mGluR-mediated calcium homeostasis in auditory neurons
Autore:
Zirpel, L; Parks, TN;
Indirizzi:
Univ Utah, Sch Med, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132 Anat, Salt Lake City, UT 84132 USA
Titolo Testata:
JARO
fascicolo: 2, volume: 2, anno: 2001,
pagine: 180 - 187
SICI:
1525-3961(200106)2:2<180:ZIOGIM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
AVIAN COCHLEAR NUCLEUS; METABOTROPIC GLUTAMATE-RECEPTOR; BRAIN-STEM; HIPPOCAMPAL-NEURONS; AMPA RECEPTORS; CELL-DEATH; RAT; ANTAGONIST; ACTIVATION; CHICKEN;
Keywords:
chick nucleus magnocellularis; metabotropic glutamate receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Zirpel, L Univ Utah, Sch Med, Dept Neurobiol & Anat, Bldg 530,Room 417,50 N Med Dr, Salt Lake City, UT 84132 USA Univ Utah Bldg 530,Room 417,50 N MedDr Salt Lake City UT USA 84132
Citazione:
L. Zirpel e T.N. Parks, "Zinc inhibition of group I mGluR-mediated calcium homeostasis in auditory neurons", JARO, 2(2), 2001, pp. 180-187

Abstract

Zinc is widely distributed in the central nervous system (CNS), it functions normally as a synaptic modulator, and it contributes to neuronal death under pathologic conditions. Zinc colocalizes with glutamate in excitatory synapses, and the presence of zinc is well characterized in the synapses of the auditory system. Since chick cochlear nucleus neurons depend upon synaptic activation of metabotropic glutamate receptors (mGluRs) for maintenanceand survival, the goal of this study was to determine (1) if zinc is released from the eighth nerve calyces onto nucleus magnocellularis (NM) neuronsin the chick cochlear nucleus, and, if so, (2) what effect it has on groupI mGluR-mediated calcium homeostasis of these neurons. Using in vitro slices and a fluorescent dye relatively specific to vesicularized zinc, we showthat zinc is indeed localized to the presynaptic calyces and is released upon nerve stimulation or KCI depolarization. Experiments employing fura-2 calcium imaging show that zinc inhibits group I mGluR release of calcium from internal stores of NM neurons and disrupts activity-dependent calcium homeostasis in a manner identical to the mGluR5-specific antagonist 2-methyl-6-(phenylethynyl) pyridine. The mGluR1-specific antagonist 7-hydroxyiminocyclopropan- [b]chromen-1a-carboxylic acid ethyl eater did not affect release of calcium from stores by the nonspecific mGluR agonist aminocyclopentane dicarboxylic acid, nor did it affect activity-dependent calcium homeostasis. We conclude that zinc is present in and released from the glutamatergic eighth nerve calyces. The presence of zinc inhibits mGluR5, a major componentof calcium homeostasis of NM neurons, and plays a modulatory role in the activity-dependent, mGluR-mediated calcium homeostasis of auditory neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:14:58