Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Screening of differentially expressed genes in 5-fluorouracil-resistant human gastrointestinal tumor cells
Autore:
Takechi, T; Koizumi, K; Tsujimoto, H; Fukushima, M;
Indirizzi:
Taisho Pharmaceut Co Ltd, Canc Res Lab 2, Hanno, Saitama 3578527, Japan Taisho Pharmaceut Co Ltd Hanno Saitama Japan 3578527 itama 3578527, Japan
Titolo Testata:
JAPANESE JOURNAL OF CANCER RESEARCH
fascicolo: 6, volume: 92, anno: 2001,
pagine: 696 - 703
SICI:
0910-5050(200106)92:6<696:SODEGI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEIN; NUCLEOSIDE TRANSPORTERS; MULTIDRUG RESISTANCE; CANCER CELLS; LINES; DNA; GEMCITABINE;
Keywords:
resistance to 5-fluorouracil; profiling of gene expression; human gastrointestinal tumor cells; mRNA differential display; northern hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Takechi, T Taisho Pharmaceut Co Ltd, Canc Res Lab 2, 1-27 Misugidai, Hanno, Saitama 3578527, Japan Taisho Pharmaceut Co Ltd 1-27 Misugidai Hanno Saitama Japan 3578527
Citazione:
T. Takechi et al., "Screening of differentially expressed genes in 5-fluorouracil-resistant human gastrointestinal tumor cells", JPN J CANC, 92(6), 2001, pp. 696-703

Abstract

To identify genes differentially expressed in association with resistance to 5-fluorouracil (5FU), an mRNA differential display (DD) analysis was used to compare transcripts from the NUGC-3 human gastric tumor cell line and the NUGC-3/5FU/L line, which had acquired 208-fold resistance as a consequence of repeated exposure to escalating concentrations of 5FU, The 110 cDNA fragments differentially expressed in the DD analysis of either the NUGC-3 or NUGC-3/5FU/L cells were sequenced and subjected to a homology search, and 29 overexpressed and 22 underespressed genes Mere identified in NUGC-3/5FU/L as a result, To confirm whether the changes in the gene expression levels in the NUGC-3/5FU/L cells were shared by other 5FU-resistant cells, 35 genes were analyzed by northern hybridization in 3 pairs of parent/5FU-resistant human gastrointestinal tumor cell lints, The analysis revealed 20 overexpressed and 10 underexpressed genes in at least one of the three 5FU-resistant cells as compared with those in the parent cells, Among them, P-glycoprotein, equilibrative nucleoside transporter 1, and methylenetetrahydrofolate dehydrogenase were highly expressed in two of the three 5FU-resistant cells and cytidine deaminase and integrin alpha3 were underespressed, The acquisition of resistance to 5FU by. tumor cells may result from multiple changes in cellular functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:36:44