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Titolo:
Examination of the oncogenic potential of H19 gene in HeLa x normal human fibroblast hybrid cells
Autore:
Tsujimoto, H; Nishizuka, S; Redpath, LJ; Stanbridge, EJ;
Indirizzi:
Univ Calif Irvine, Coll Med, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 l & Mol Genet, Irvine, CA 92697 USA Univ Calif Irvine, Coll Med, Div Radiat Oncol, Dept Radiol Sci, Irvine, CA92697 USA Univ Calif Irvine Irvine CA USA 92697 ept Radiol Sci, Irvine, CA92697 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 1, volume: 19, anno: 2001,
pagine: 89 - 95
SICI:
1019-6439(200107)19:1<89:EOTOPO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL ALKALINE-PHOSPHATASE; TUMOR-ASSOCIATED ANTIGEN; IGF-2 GENES; EXPRESSION; TUMORIGENICITY; CHROMOSOME-11; SUPPRESSION; RNA; TRANSFORMATION;
Keywords:
H19 gene; HeLa cell; hybrid cell; tumorigenicity; transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Tsujimoto, H Shiga Univ Med Sci, Dept Pathol 1, Ohtsu, Shiga 5202192, Japan Shiga Univ Med Sci Ohtsu Shiga Japan 5202192 5202192, Japan
Citazione:
H. Tsujimoto et al., "Examination of the oncogenic potential of H19 gene in HeLa x normal human fibroblast hybrid cells", INT J ONCOL, 19(1), 2001, pp. 89-95

Abstract

Fusion of tumorigenic HeLa cells with human skin fibroblasts results in chromosomally stable non-tumorigenic hybrids. The studies of rare spontaneoustumorigenic segregants from the non-tumorigenic hybrid have implicated theloss of one copy of human fibroblast chromosome 11 with concomitant re-expression of tumorigenicity. In a previous study of differential display screening, we reported the tumorigenic-specific expression of H19 gene as a possible candidate for elicitation of tumorigenic phenotype. In this study, weexamined the expression of H19 gene using gamma -ray-induced tumorigenic mutants (GIMs) and non-tumorigenic irradiated control cells (CONs) from the non-tumorigenic hybrids. H19 expression was recognized in all the GIMs withvarious expression levels, whereas no CONs expressed the H19 gene. To examine the tumorigenic potentials of H19 gene directly, we introduced an H19 gene expression vector into the non-tumorigenic hybrids and assayed the tumorigenicity of the transfectants by s.c. injection into athymic nude mice. However, no transfectants with stable H19 gene expression induced in vivo tumor growth. These results suggest that expression of the H19 gene may be necessary but is not sufficient to confer the tumorigenic phenotype in HeLa xfibroblast hybrids.

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Documento generato il 27/09/20 alle ore 19:08:58