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Titolo:
Expression of galectin-3-reactive ligands in squamous cancer and normal epithelial cells as a marker of differentiation
Autore:
Plzak, J; Smetana, K; Hrdlickova, E; Kodet, R; Holikova, Z; Liu, FT; Dvorankova, B; Kaltner, H; Betka, J; Gabius, HJ;
Indirizzi:
Charles Univ, Fac Med 1, Inst Anat, Prague 12800 2, Czech Republic CharlesUniv Prague Czech Republic 12800 2 rague 12800 2, Czech Republic Charles Univ, Fac Med 1, Dept Otorhinolaryngol Head & Neck Surg, Prague 12800 2, Czech Republic Charles Univ Prague Czech Republic 12800 2 rague 12800 2, Czech Republic Charles Univ, Fac Med 2, Ctr Cell Therapy & Tissue Repair, Prague 12800 2,Czech Republic Charles Univ Prague Czech Republic 12800 2 Prague 12800 2,Czech Republic Charles Univ, Fac Med 1, Dept Ophthalmol, Prague 12800 2, Czech Republic Charles Univ Prague Czech Republic 12800 2 rague 12800 2, Czech Republic Charles Univ, Fac Med 2, Inst Pathol Anat, Prague 12800 2, Czech Republic Charles Univ Prague Czech Republic 12800 2 rague 12800 2, Czech Republic Charles Univ, Fac Med 2, Dept Dermatol, Prague 12800 2, Czech Republic Charles Univ Prague Czech Republic 12800 2 rague 12800 2, Czech Republic La Jolla Inst Allergy & Immunol, San Diego, CA USA La Jolla Inst Allergy &Immunol San Diego CA USA unol, San Diego, CA USA Charles Univ, Fac Med 3, Prague Burn Ctr, Prague, Czech Republic Charles Univ Prague Czech Republic gue Burn Ctr, Prague, Czech Republic Univ Munich, Fac Vet Med, Inst Physiol Chem, Munich, Germany Univ Munich Munich Germany Vet Med, Inst Physiol Chem, Munich, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 1, volume: 19, anno: 2001,
pagine: 59 - 64
SICI:
1019-6439(200107)19:1<59:EOGLIS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSIDE-BINDING LECTINS; ENDOGENOUS LECTINS; ANIMAL LECTINS; TERMINAL DIFFERENTIATION; GALECTINS-1 AND-3; HUMAN CARCINOMAS; TUMOR BIOLOGY; KERATINOCYTES; MODULATION; MOLECULES;
Keywords:
galectin; lectin histochemistry; head and neck cancer; larynx; oropharynx; squamous cell cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Smetana, K Charles Univ, Fac Med 1, Inst Anat, U Nemocnice 3, Prague 128002, Czech Republic Charles Univ U Nemocnice 3 Prague Czech Republic 12800 2 ublic
Citazione:
J. Plzak et al., "Expression of galectin-3-reactive ligands in squamous cancer and normal epithelial cells as a marker of differentiation", INT J ONCOL, 19(1), 2001, pp. 59-64

Abstract

The definition of biological markers for oropharynx and larynx cancer is essential to predict their clinical behavior. Since cellular glycans play animportant role in biological information transfer, we have employed an endogenous lectin, galectin-3, to examine in primary squamous carcinomas, lymph node metastases, and physiological squamous epithelia whether glycans recognized by this lectin are altered in relation to the state of differentiation. The expression of galectin-3 was concomitantly evaluated by immunohistochemistry using the A1D6 monoclonal antibody. In addition, other antibodies were used for the detection of cytokeratins and desmosomal proteins (desmoplakin-l and desmoglein). The results show the expression of galectin-3-reactive ligands in moderately/highly differentiated carcinomas only in areasexhibiting a high level of keratinization. Except for one patient out of 14, metastatic cells in lymph nodes expressed no accessible binding sites for galectin-3. No galectin-3-reactivity was detected in the basal cell layerof all studied normal epithelia (which contains the proliferating cells). The suprabasal layers were positive in epidermis and epithelium of tongue and cornea and negative in epithelium of palatine tonsil. The tumor cells expressed galectin-3 with an intensity positively correlated with tumor differentiation. The position of galectin-3-reactive sites colocalized with the two tested desmosomal proteins. However, presence of these proteins was also detected in areas of tumor and suprabasal layers of tonsil epithelium where no binding reactivity fur galecrin-3 was found. The present study showedthat expression of galectin-3-reactive glycoligands is differentiation-dependent in normal as well as malignant squamous cells. Colocalization of galectin-3-reactive sites with desmosomal proteins (desmoplakin-1 and desmoglein) suggests an association of the galectin-3 ligand(s) with the cell surface, pointing to a potential participation of galectin-3 in mediation of intercellular contacts in these tumor types.

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Documento generato il 27/09/20 alle ore 06:04:26