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Titolo:
Unchanged frequency of loss of heterozygosity and size of the deleted region at 8p21-23 during metastasis of lung cancer
Autore:
Kurimoto, F; Gemma, A; Hosoya, Y; Seike, M; Takenaka, K; Uematsu, K; Yoshimura, A; Shibuya, M; Kudoh, S;
Indirizzi:
Nippon Med Sch, Dept Internal Med 4, Bunkyo Ku, Tokyo 1138602, Japan Nippon Med Sch Tokyo Japan 1138602 ed 4, Bunkyo Ku, Tokyo 1138602, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 1, volume: 8, anno: 2001,
pagine: 89 - 93
SICI:
1107-3756(200107)8:1<89:UFOLOH>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALLELIC LOSS; HEPATOCELLULAR-CARCINOMA; MOLECULAR ABNORMALITIES; PRENEOPLASTIC LESIONS; BRONCHIAL EPITHELIUM; COLORECTAL-CANCER; CHROMOSOME 8P; FHIT GENE; SHORT ARM; CELL;
Keywords:
LOH; lung cancer; chromosome 8p; metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Gemma, A Nippon Med Sch, Dept Internal Med 4, Bunkyo Ku, 1-1-5 Sendagi, Tokyo 113, Japan Nippon Med Sch 1-1-5 Sendagi Tokyo Japan 113 i, Tokyo 113, Japan
Citazione:
F. Kurimoto et al., "Unchanged frequency of loss of heterozygosity and size of the deleted region at 8p21-23 during metastasis of lung cancer", INT J MOL M, 8(1), 2001, pp. 89-93

Abstract

The genetic mechanisms involved in lung cancer development and progressionare beginning to be understood. Many studies have documented frequent lossof heterozygosity (LOH) at specific chromosomal regions in cancer cells; this implies that turner suppressor genes (TSG) are usually present in thoseregions. Recently, it has been reported that LOH or chromosomal deletions at chromosome 8p21-23 represent early events frequently occurring in lung cancer. In addition, the size of these chromosome 8 deletions, as well as their frequency, was also reported to increase during lung cancer progression. To determine the spectrum and frequency of alterations of chromosome 8p21-23 in human lung cancer and whether these increase with progression of thetumors, we performed LOH analysis of chromosome 8p and 3p in the genomic DNA from cells from primary and metastatic sites of lung cancer, as well as from normal lung. We studied 35 subjects with primary lung cancer including30 tumors with distant metastasis. Detection of allelic deletion utilized a PCR-based approach of microsatellite polymorphism analysis, which was performed using the microsatellite markers D8S1130, D8S1106, D8S511, D8S1827, D8S549, D8S261, LPL, D8S258, D8S136, NEFL, D351295, D3S1313, D3S1234, D3S1300, D351351, D3S1339, and D3S1340. The overall allelic deletion rates were 10 of 28 (35.7%) at 8p and 13 of 33 (39.4%) at 3p. The allelic deletions inthe primary cancer and its metastatic sites were in each case identical inboth frequency and size of the deleted regions. In our analysis, 8p21-23 deletions were not always associated with 3p deletions in primary lung cancer. These results therefore suggest that allelic deletion at chromosome 8p21-23 is an early and frequent event in the carcinogenesis and development oflung cancer, independent of chromosome 3p deletion. However, a continuing increase in the frequency of LOH at 8p21-23 and in the size of the deleted region rarely occurs during the process of metastasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:33:28