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Titolo:
Murine M2-10B4 and SL/SL cell lines differentially affect the balance between CD34(+) cell expansion and maturation
Autore:
Koschmieder, S; Bug, G; Schroder, B; Rossmanith, T; Hofmann, WK; Kalina, U; Hoelzer, D; Ottmann, OG;
Indirizzi:
Johann Wolfgang Goethe Univ Hosp, Dept Internal Med 3, Div Hematol, Frankfurt, Germany Johann Wolfgang Goethe Univ Hosp Frankfurt Germany , Frankfurt, Germany MainGen Biotechnol GmbH, Frankfurt, Germany MainGen Biotechnol GmbH Frankfurt Germany hnol GmbH, Frankfurt, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF HEMATOLOGY
fascicolo: 1, volume: 73, anno: 2001,
pagine: 71 - 77
SICI:
0925-5710(200101)73:1<71:MMASCL>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; EX-VIVO EXPANSION; HEMATOPOIETIC PROGENITOR CELLS; GROWTH-FACTOR-BETA; STROMAL CELLS; FLT3 LIGAND; STEM-CELLS; THROMBOPOIETIN; INTERLEUKIN-3; APOPTOSIS;
Keywords:
CD34(+) cells; M2-10B4; Sl/Sl; bone marrow stroma; stem cell expansion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Koschmieder, S Med Klin 3, Klin Hamatol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany Med Klin 3 Theodor Stern Kai 7 Frankfurt Germany D-60590 y
Citazione:
S. Koschmieder et al., "Murine M2-10B4 and SL/SL cell lines differentially affect the balance between CD34(+) cell expansion and maturation", INT J HEMAT, 73(1), 2001, pp. 71-77

Abstract

The ability of bone marrow stroma to modulate hematopoietic progenitor cell expansion is of considerable interest for gene transfer strategies and transplantation of limited stem cell numbers. We compared the capacity of 2 murine stromal cell lines to affect the balance between maturation and proliferation of human CD34(+) cells in short-term expansion cultures. In 7-day serum-free cultures, cytokine-induced amplification of granulocyte-macrophage colony-forming cells (CFC-GM), erythroid burst-forming units (BFU-E), and total cells was significantly increased by the presence of genetically engineered S1/S1 and M2-10B4 stromal cells in a 1:1 ratio (S1/M2 cells) compared with stroma-free cultures (P < .05). S1/M2 cultures generated 21-fold more mature CD15(+) cells than stroma-free cultures, without further amplifying the number of CD34(+) cells. The addition of serum led to a further increase of CFC-GM total cells, and CD15(+) cells, whereas BFU-E were no longer maintained. Pure S1/S1 stromal layers were likewise superior to stroma-free cultures in expansion of CD34(+) cells and total cells when serum was present. However, the differentiation of CD34(+) cells was less pronounced inS1/S1 cultures compared with S1/M2 layers, as demonstrated by a lower content of CD15(+) cells. Neutralization experiments revealed differential contributions of Flt3 ligand and thrombopoietin to the support of total cell and CFC expansion by S1/M2 and S1/S1 stromal feeders. (C) 2001 The Japanese Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:36:36