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Titolo:
Ex vivo expansion of human hematopoietic stem cells
Autore:
Nakahata, T;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Pediat, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 pt Pediat, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF HEMATOLOGY
fascicolo: 1, volume: 73, anno: 2001,
pagine: 6 - 13
SICI:
0925-5710(200101)73:1<6:EVEOHH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CD34(+) CELLS; CORD-BLOOD; BONE-MARROW; IL-6 RECEPTOR; SELF-RENEWAL; IN-VITRO; REPOPULATING CELLS; FLK2/FLT3 LIGAND; TYROSINE KINASE; GP130;
Keywords:
hematopoietic stem cells; ex vivo expansion; Il-6/sIL-6R complex; NOD/Shi-SCID mouse; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Nakahata, T Kyoto Univ, Grad Sch Med, Dept Pediat, Sakyo Ku, 54 Kawaharacho, Kyoto 6068507, Japan Kyoto Univ 54 Kawaharacho Kyoto Japan 6068507 6068507, Japan
Citazione:
T. Nakahata, "Ex vivo expansion of human hematopoietic stem cells", INT J HEMAT, 73(1), 2001, pp. 6-13

Abstract

There has been great interest in the ex vivo expansion of human long term repopulating hematopoietic stem cells (LTR-HSCs) for a variety of clinical applications such as umbilical cord blood transplantation. The glucoprotein130 signal, activated by a complex of interleukin 6 (IL-6) and soluble IL-6 receptor (IL-6l/sIL-6R), acts dramatically in synergy with the c-Kit or Flk2/Flt3 signal to expand immature human HSCs, We demonstrate a significantex vivo expansion of human LTR-HSCs capable of repopulating in newly discovered nonobese diabetes/Shi-severe combined immunodeficient (NOD/Shi-SCID) mice. The proportion of human CD45-cells in recipient marrow was 10 times higher in animals receiving the cultured cells with stem cell factor, Flk2/Flt3 ligand, thrombopoietin, and IL-6/sIL-6R than in those receiving comparable numbers of fresh cord blood CD34(+) cells. The expansion rate provided by this combination was estimated to be 4.2-fold by a limiting dilution method, Addition of IL-3 to the culture with the cytokine combination abrogated the repopulating ability of the expanded cells. The culture method with the IL-6/sIL-6R complex and other cytokines may pave the way for ex vivo expansion of human transplantable (C) 2001 The Japanese Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:03:25