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Titolo:
Microsatellite loci are not abundant in all arthropod genomes: analyses inthe hard tick, Ixodes scapularis and the yellow fever mosquito, Aedes aegypti
Autore:
Fagerberg, AJ; Fulton, RE; Black, WC;
Indirizzi:
Colorado State Univ, Dept Microbiol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 iol, Ft Collins, CO 80523 USA
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 3, volume: 10, anno: 2001,
pagine: 225 - 236
SICI:
0962-1075(200106)10:3<225:MLANAI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTITATIVE TRAIT LOCI; SILKWORM BOMBYX-MORI; ANOPHELES-GAMBIAE; MALARIA VECTOR; PHYLOGENETIC-RELATIONSHIPS; DROSOPHILA-MELANOGASTER; GENETIC DIFFERENTIATION; DNA POLYMORPHISMS; WEST-AFRICA; SOCIAL WASP;
Keywords:
microsatellites; enriched librarie; Ixodes scapularis; Aedes aegypti;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Black, WC Colorado State Univ, Dept Microbiol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 llins, CO 80523 USA
Citazione:
A.J. Fagerberg et al., "Microsatellite loci are not abundant in all arthropod genomes: analyses inthe hard tick, Ixodes scapularis and the yellow fever mosquito, Aedes aegypti", INSEC MOL B, 10(3), 2001, pp. 225-236

Abstract

Plasmid libraries enriched for microsatellites were generated in the tick,Ixodes scapularis and in the mosquito Aedes aegypti. Libraries were enriched for genomic DNA containing (AC)(n), (AG)(n), (ATG)(n), (CAG)(n), (TAG)(n), (AAT)(n), (CTGY)(n) or (GATA)(n) motifs. Clones containing each motif were sequenced in both species for PCR primer design. In I. scapularis, most primers amplified a single locus and alleles varied in the number of microsatellite repeats and segregated as codominant markers. In contrast (AC)(n),(TAG)(n) and (GATA)(n) microsatellite loci extracted from Ae. aegypti appeared to be members of multigene families. A primer pair designed to amplifya particular TAG locus instead amplified many independently segregating loci, some of which did not contain TAG microsatellites. Alleles at the TAG loci segregated as dominant markers and there was limited evidence for length variation among alleles. These results suggest that microsatellite loci are not universally abundant in arthropod genomes nor do alleles always segregate as codominant markers.

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Documento generato il 09/07/20 alle ore 01:39:56