Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
False positive reaction in ELISA for IgM class anti-M2 antibody and its prevention
Autore:
Miyakawa, H; Kawaguchi, N; Kikuchi, K; Kitazawa, E; Kawashima, Y; Yajima, R; Itoh, Y;
Indirizzi:
Teikyo Univ, Sch Med, Dept Internal Med 4, Takatsu Ku, Kawasaki, Kanagawa 2138507, Japan Teikyo Univ Kawasaki Kanagawa Japan 2138507 saki, Kanagawa 2138507, Japan Inst Immunol, Bunkyo Ku, Tokyo 1120004, Japan Inst Immunol Tokyo Japan 1120004 mmunol, Bunkyo Ku, Tokyo 1120004, Japan
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 3, volume: 20, anno: 2001,
pagine: 279 - 287
SICI:
1386-6346(200107)20:3<279:FPRIEF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY BILIARY-CIRRHOSIS; SERUM-PROTEINS; AUTOANTIBODIES; DISORDERS; ASSAY;
Keywords:
enzyme-linked immunosorbent assay (ELISA); non-specific binding; antibodies blocking reagent; anti-M2; primary biliary cirrhosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Miyakawa, H Teikyo Univ, Sch Med, Dept Internal Med 4, Takatsu Ku, 3-8-3 Mizonokuchi, Kawasaki, Kanagawa 2138507, Japan Teikyo Univ 3-8-3 MizonokuchiKawasaki Kanagawa Japan 2138507
Citazione:
H. Miyakawa et al., "False positive reaction in ELISA for IgM class anti-M2 antibody and its prevention", HEPATOL RES, 20(3), 2001, pp. 279-287

Abstract

Anti-MZ of anti-mitochondrial antibodies is recognized as the specific autoantibody detected in sera from patients with primary biliary cirrhosis (PBC). The IgG class and IgM class of this antibody can be separately measuredusing each ELISA. In the present study, false positive reactions were found in some sera from non-PBC patients such as acute hepatitis A, syphilis and rheumatoid arthritis using the IgM anti-M2 ELISA. They showed an increaseof polyclonal IgM, and positivity for IgM anti-cardiolipin or rheumatoid factors, respectively. So, we developed a means to prevent these false positive reactions. First, dilutions of test sera at 1:1000-fold were carried out in addition to the original method at 1:100-fold. Secondly, some blockingreagents were added into the buffer system. By serum dilution, non-specific bindings disappeared in most samples other than showing an increase in polyclonal IgM. Moreover, the addition of suitable blocking reagents such as fetal bovine serum (FBS) and skimmed milk into the buffer system could prevent these non-specific bindings. From these findings, the procedure of optical serum dilution and the addition of suitable blocking reagents successfully prevented false positive reactions in this IgM anti-M2 ELISA. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:04:35