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Titolo:
Inclusion of the herpes simplex thymidine kinase gene in a replicating adenovirus does not augment antitumor efficacy
Autore:
Lambright, ES; Amin, K; Wiewrodt, R; Force, SD; Lanuti, M; Propert, KJ; Litzky, L; Kaiser, LR; Albelda, SM;
Indirizzi:
Univ Penn, Ctr Med, Dept Med, Pulm Allergy & Crit Care Div, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Care Div, Philadelphia, PA 19104 USA Univ Penn, Ctr Med, Dept Surg, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dept Surg, Philadelphia, PA 19104 USA Univ Penn, Ctr Med, Dept Biostat, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Biostat, Philadelphia, PA 19104 USA Univ Penn, Ctr Med, Dept Pathol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 pt Pathol, Philadelphia, PA 19104 USA
Titolo Testata:
GENE THERAPY
fascicolo: 12, volume: 8, anno: 2001,
pagine: 946 - 953
SICI:
0969-7128(200106)8:12<946:IOTHST>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MALIGNANT MESOTHELIOMA; DOUBLE SUICIDE GENE; I CLINICAL-TRIAL; CANCER-CELLS; ONCOLYTIC ADENOVIRUS; LUNG-CANCER; THERAPY; AGENTS; VIVO; DELIVERY;
Keywords:
herpes simplex thymidine kinase; adenovirus; replicating adenovirus; gene therapy; cancer gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Albelda, SM Univ Penn, Ctr Med, Dept Med, Pulm Allergy & Crit Care Div, 856 BRB2-3,421Curie Blvd, Philadelphia, PA 19104 USA Univ Penn 856 BRB2-3,421Curie Blvd Philadelphia PA USA 19104
Citazione:
E.S. Lambright et al., "Inclusion of the herpes simplex thymidine kinase gene in a replicating adenovirus does not augment antitumor efficacy", GENE THER, 8(12), 2001, pp. 946-953

Abstract

Replication-incompetent adenoviruses (Ad) carrying the herpes simplex thymidine kinase (HSVtk) gene have been used in a number of human cancer gene therapy trials, however transduction has generally been limited to a small minority of tumor cells. To solve this problem, replication-competent adenoviral Vectors carrying transgenes such as HSVtk have been developed. However, contradictory evidence exists regarding the efficacy of these new vectors. Accordingly, we constructed and tested a replication-competent E3-deletedadenoviral vector containing the HSVtk suicide gene driven by the endogenous E3 promoter (Ad.wt.tk). This virus showed high level production of the HSVtk transgene and was more efficacious than a non-replicating virus in vitro, after injection into flank tumors, and against established intraperitoneal tumors. However, addition of ganciclovir (GCV) therapy to cells or tumor-bearing animals treated with the replicating vector containing the HSVtk suicide gene did not result in increased cell killing. Our results indicatethat addition of HSVtk to a replicating Ad Virus will not likely be usefulin augmenting antitumor effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:53:26