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Titolo:
Streptomyces matensis laminaripentaose hydrolase is an 'inverting' beta-1,3-glucanase
Autore:
Nishimura, T; Bignon, C; Allouch, J; Czjzek, M; Darbon, H; Watanabe, T; Henrissat, B;
Indirizzi:
Univ Aix Marseille 1, CNRS, F-13402 Marseille 20, France Univ Aix Marseille 1 Marseille France 20 S, F-13402 Marseille 20, France Univ Aix Marseille 2, CNRS, F-13402 Marseille, France Univ Aix Marseille 2 Marseille France F-13402 F-13402 Marseille, France Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 95021, Japan Niigata Univ Niigata Japan 95021 pt Appl Biol Chem, Niigata 95021, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 1-2, volume: 499, anno: 2001,
pagine: 187 - 190
SICI:
0014-5793(20010615)499:1-2<187:SMLHIA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-SEQUENCE SIMILARITIES; GLYCOSYL HYDROLASES; STEREOCHEMICAL COURSE; HYDROLYSIS; CLASSIFICATION; MECHANISMS;
Keywords:
beta-1,3-glucanase; laminaripentaose hydrolase; reaction mechanism; Streptomyces matensis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Henrissat, B Univ Aix Marseille 1, CNRS, 31 Chemin Joseph Aiguier, F-13402Marseille 20, France Univ Aix Marseille 1 31 Chemin Joseph Aiguier Marseille France 20
Citazione:
T. Nishimura et al., "Streptomyces matensis laminaripentaose hydrolase is an 'inverting' beta-1,3-glucanase", FEBS LETTER, 499(1-2), 2001, pp. 187-190

Abstract

The laminaripentaose-producing beta -1,3-glucanase of Streptomyces matensis is a member of the glycoside hydrolase family GH-64. We have constructed and purified a recombinant hexahistidine-tagged form of the enzyme for characterisation, The enzyme, which exists as a monomer in solution, hydrolysisbeta -1,3-glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH-64 and this is the first characterisation of an 'inverting' beta -1,3-glucanase. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B,V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:59:37