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Titolo:
Melatonin attenuates estradiol-induced oxidative damage to DNA: Relevance for cancer prevention
Autore:
Karbownik, M; Reiter, RJ; Burkhardt, S; Gitto, E; Tan, DX; Lewinski, A;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 truct Biol, San Antonio, TX 78229 USA Med Univ Lodz, Inst Endocrinol, Dept Thyroidol, PL-91425 Lodz, Poland Med Univ Lodz Lodz Poland PL-91425 Dept Thyroidol, PL-91425 Lodz, Poland
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 7, volume: 226, anno: 2001,
pagine: 707 - 712
SICI:
1535-3702(200107)226:7<707:MAEODT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSOMAL MEMBRANE FLUIDITY; INDUCED LIPID-PEROXIDATION; MALE SYRIAN-HAMSTERS; PINEAL HORMONE; IN-VIVO; ENDOMETRIAL CANCER; FREE-RADICALS; NITRIC-OXIDE; ESTROGEN; CELL;
Keywords:
DNA damage; lipid peroxidation; kidney; liver; melatonin; estrogen; hamster;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Reiter, RJ Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, Mail Code 7762,7703Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas Mail Code 7762,7703 Floyd Curl Dr San Antonio TX USA 78229
Citazione:
M. Karbownik et al., "Melatonin attenuates estradiol-induced oxidative damage to DNA: Relevance for cancer prevention", EXP BIOL ME, 226(7), 2001, pp. 707-712

Abstract

Estrogens exert pro-oxidative effects and have been shown to damage DNA, potentially leading to cancer. Melatonin is a well-known antioxidant, free radical scavenger, and oncostatic agent. Changes in the levels of 8-oxo-7,8-dihydro-2 ' -deoxyguanosine (8-oxodGuo), an index of DNA damage, and the levels of malondialdehyde + 4-hydroxyalkenals, an index of lipid peroxidation, were measured in kidneys, liver, and testes from hamsters treated with E-2 (75 mg/kg body wt) and were collected 3 or 5 hr later. Other animals weretreated with melatonin (15 mg/kg body wt, 30 min before and 120 min after E-2 treatment) or were given both compounds. Additionally, lipid peroxidation was measured in liver homogenates exposed to ferrous sulfate (15 muM) invitro. E-2 treatment caused an increase in 8-oxodGuo levels in kidneys collected 5 hr after E-2 administration, and in liver 3 hr after estrogen treatment. Melatonin completely prevented E-2-induced DNA damage in both organs. Melatonin alone or when given with E-2 and examined 3 hr later decreased the base level of 8-oxodGuo in testes. A tendency for a reduction in in vivo lipid peroxidation was observed after treatment of hamsters with either melatonin, E-2, or both compounds, with a statistically significant decreasebeing measured in the liver following E-2 administration. In vitro exposure to iron significantly enhanced lipid peroxidation in hepatic homogenates from untreated, melatonin-treated, or E-2-injected hamsters; in the hepatichomogenates of hamsters given both E-2 and melatonin, ferrous sulfate failed to augment lipid peroxidation, Our results confirm the dual actions of estrogens relative to oxidative damage, i,e,, estrogen increases oxidative destruction of DNA while reducing lipid peroxidation, Melatonin had antioxidative actions in reducing oxidative damage to both DNA and to membrane lipids. Melatonin completely prevented the damaging action of E-2 on DNA and synergized with the steroid to reduce lipid peroxidation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:39:32