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Titolo:
Meiotic segregation analysis in a t(4;8) carrier: comparison of FISH methods on sperm chromosome metaphases and interphase sperm nuclei
Autore:
Oliver-Bonet, M; Navarro, J; Codina-Pascual, M; Carrera, M; Egozcue, J; Benet, J;
Indirizzi:
Univ Autonoma Barcelona, Unitat Biol, Fac Med, Dept Biol Cellular Fisiol &Immunol, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona SpainE-08193 E-08193 Barcelona, Spain Ctr Patol Celular, Barcelona, Spain Ctr Patol Celular Barcelona SpainCtr Patol Celular, Barcelona, Spain Autonomous Univ Barcelona, Unitat Biol Cellular, Fac Ciencies, Dept Biol Cellular Fisiol & Immunol, E-08193 Barcelona, Spain Autonomous Univ Barcelona Barcelona Spain E-08193 08193 Barcelona, Spain
Titolo Testata:
EUROPEAN JOURNAL OF HUMAN GENETICS
fascicolo: 6, volume: 9, anno: 2001,
pagine: 395 - 403
SICI:
1018-4813(200106)9:6<395:MSAIAT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU-HYBRIDIZATION; RECIPROCAL TRANSLOCATION T(11-22)(Q23-Q11); MALE-INFERTILITY; SPERMATOZOA; MEN; REARRANGEMENTS; ABNORMALITIES; ANEUPLOIDY; CYTOGENETICS; COMPLEMENTS;
Keywords:
sperm chromosome; sperm nuclei; meiotic segregation; chromosome rearrangements; aneuploidy; FISH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Oliver-Bonet, M Univ Autonoma Barcelona, Unitat Biol, Fac Med, Dept Biol Cellular Fisiol &Immunol, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 a, Spain
Citazione:
M. Oliver-Bonet et al., "Meiotic segregation analysis in a t(4;8) carrier: comparison of FISH methods on sperm chromosome metaphases and interphase sperm nuclei", EUR J HUM G, 9(6), 2001, pp. 395-403

Abstract

Meiotic segregation of a t(4;8)(q28;p23) translocation carrier was determined by two different methods to compare the final results. A total of 352 sperm chromosome complements, obtained after human-hamster in vitro fertilisation, were analysed by whole chromosome painting, and 6590 sperm heads were studied by fluorescence in situ hybridisation (FISH). Frequencies of alternate, adjacent I, adjacent II and 3:1 segregations were, for sperm chromosomes, 35.5, 33.2, 19.9 and 11.3% respectively. For sperm head analysis, results were 30.5, 28.5, 20.5 and 19.5% respectively. There were no statistically significant differences between the two methods with respect to the observed frequencies of sperm with balanced and unbalanced chromosome constitutions. Among unbalanced gametes, the methods differed only in the frequencyof 3:1 segregation (chi (2), P <0.0001). Different factors that could explain this result are discussed. To determine possible interchromosomal effects, multicolour FISH was used on sperm heads. Disomy rates of sex and 18 chromosomes were higher in the translocation carrier than in the control. Thedifferences observed were statistically significant (P <0.0001 for chromosomes X and 18, and P=0.0091 for chromosome Y).

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Documento generato il 20/09/20 alle ore 01:07:32