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Titolo:
Changes in the levels of low-abundance brain proteins induced by kainic acid
Autore:
Krapfenbauer, K; Berger, M; Friedlein, A; Lubec, G; Fountoulakis, M;
Indirizzi:
F Hoffmann La Roche & Co Ltd, Pharmaceut Res, Genome Technol, CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4070 asel, Switzerland Univ Vienna, Dept Pediat, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 a, Dept Pediat, A-1010 Vienna, Austria Univ Vienna, Inst Brain Res, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 Inst Brain Res, A-1010 Vienna, Austria
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 12, volume: 268, anno: 2001,
pagine: 3532 - 3537
SICI:
0014-2956(200106)268:12<3532:CITLOL>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPARIN-BINDING PROTEIN; DEVELOPING RAT-BRAIN; HEAT-SHOCK PROTEIN; STATUS EPILEPTICUS; DNA FRAGMENTATION; EXPRESSION; SEQUENCE; CELLS; IDENTIFICATION; LOCALIZATION;
Keywords:
brain; kainic acid; low-abundance proteins; proteomics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Fountoulakis, M F Hoffmann La Roche & Co Ltd, Pharmaceut Res, Genome Technol, Bldg 93-444,CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Bldg 93-444 Basel Switzerland CH-4070
Citazione:
K. Krapfenbauer et al., "Changes in the levels of low-abundance brain proteins induced by kainic acid", EUR J BIOCH, 268(12), 2001, pp. 3532-3537

Abstract

Low-abundance gene products are of interest in proteomic studies, because they are probably involved in disease-related changes and their altered levels or modifications may carry significant biological information. Detection of low-abundance proteins of a proteome is one of the major limitations of proteomics and a scientific challenge. We investigated the changes in thelevels of low-abundance rat brain cytosolic proteins after administration of kainic acid, a potent neurotoxin and excitatory amino acid. The cytosolic proteins from controls and animals treated with kainic acid were fractionated on an ion-exchange column. The fractions collected were analyzed by 2Delectrophoresis, and the proteins with altered levels were identified by matrix-assisted laser desorption ionization or ion-spray MS. We found a manifold decrease in annexin VII, heat-shock cofactor HOP/p60 and SP-22 and a manifold increase in heparin-binding protein p30. The results suggest, respectively, the involvement of an apoptotic pathway, recruitment of the heat-shock protein machinery, generation of an antioxidant response, and, probably, induction of repair mechanisms. Three of the four proteins with altered levels had not been previously detected in the cytosolic fraction, and detection of the altered levels was possible only after the protein-enriching step.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:52:23