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Titolo:
Recognition of polyadenylation sites in yeast pre-mRNAs by cleavage and polyadenylation factor
Autore:
Dichtl, B; Keller, W;
Indirizzi:
Univ Basel, Biozentrum, Dept Cell Biol, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 ll Biol, CH-4056 Basel, Switzerland
Titolo Testata:
EMBO JOURNAL
fascicolo: 12, volume: 20, anno: 2001,
pagine: 3197 - 3209
SICI:
0261-4189(20010615)20:12<3197:ROPSIY>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA 3'-END FORMATION; MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; SPECIFICITY FACTOR; BINDING PROTEIN; EFFICIENCY; SUBUNIT; IDENTIFICATION; SEPARATION; COMPONENTS;
Keywords:
mRNA; polyadenylation signals; pre-mRNA 3 '-end processing; RNA-protein interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Keller, W Univ Basel, Biozentrum, Dept Cell Biol, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 H-4056 Basel, Switzerland
Citazione:
B. Dichtl e W. Keller, "Recognition of polyadenylation sites in yeast pre-mRNAs by cleavage and polyadenylation factor", EMBO J, 20(12), 2001, pp. 3197-3209

Abstract

Recognition of poly(A) sites in yeast pre-mRNAs is poorly understood, Employing an in vitro cleavage system with cleavage and polyadenylation factor (CPF) and cleavage factor IA we show that the efficiency and positioning elements are dispensable for poly(A)-site recognition within a short CYC1 substrate in vitro. Instead, U-rich elements immediately upstream and downstream of the poly(A) site mediate cleavage-site recognition within CYC1 and ADH1 pre-mRNAs, These elements act in concert with the poly(A) site to produce multiple recognition sites for the processing machinery, since combinations of mutations within these elements were most effective in cleavage inhibition. Intriguingly, introduction of a U-rich element downstream of the GAL7 poly(A) site strongly enhanced cleavage, underscoring the importance of downstream sequences in general, RNA-binding analyses demonstrate that cleavage depends on the recognition of the poly(A)-site region by CPF, Consistent with in vitro results, mutation of sequences upstream and downstream of the poly(A) site affected 3'-end formation in vivo. A model for yeast pre-mRNA cleavage-site recognition outlines an unanticipated high conservation ofyeast and mammalian 3'-end processing mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:10:07