Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of D-glucose on nitric oxide release from glomerular endothelial cells
Autore:
Kasai, N; Sugimoto, K; Horiba, N; Suda, T;
Indirizzi:
Hirosaki Univ, Sch Med, Dept Internal Med 3, Hirosaki, Aomori 036, Japan Hirosaki Univ Hirosaki Aomori Japan 036 ed 3, Hirosaki, Aomori 036, Japan
Titolo Testata:
DIABETES-METABOLISM RESEARCH AND REVIEWS
fascicolo: 3, volume: 17, anno: 2001,
pagine: 217 - 222
SICI:
1520-7552(200105/06)17:3<217:EODONO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; RELAXING FACTOR; DIABETIC COMPLICATIONS; ELEVATED GLUCOSE; ALDOSE REDUCTASE; AMBIENT GLUCOSE; FILTRATION RATE; KIDNEY SIZE; SYNTHASE; INSULIN;
Keywords:
nitric oxide; hyperglycemia; glomerular endothelial cell; diabetic nephropathy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Sugimoto, K Hirosaki Univ, Sch Med, Dept Internal Med 3, 5 Zaifu Cho, Hirosaki, Aomori036, Japan Hirosaki Univ 5 Zaifu Cho Hirosaki Aomori Japan 036 036, Japan
Citazione:
N. Kasai et al., "Effect of D-glucose on nitric oxide release from glomerular endothelial cells", DIABET M R, 17(3), 2001, pp. 217-222

Abstract

Background Altered glomerular production of nitric oxide (NO) may be involved in hyperfiltration in early diabetic nephropathy. However little is known as to the role of glomerular endothelial cells (GECs) in diabetic hyperfiltration and their ability to release NO in response to hyperglycemia. Methods Using an established cell line, we directly monitored NO release from GECs in response to various concentrations of D-glucose, D-mannitol, and L-arginine, an NO synthase (NOS) agonist. L-Arginine-induced NO release was examined in the cells pretreated for different periods up to 24 h with 10 or 30 mM D-glucose. We also measured serially the accumulation of nitrite, the stable metabolite of NO, produced by the cells incubated for up to 24h under 10 or 30 mM D-glucose conditions in the presence or absence of theNOS inhibitor, L-NAME. Results Direct measurement of NO demonstrated that D-glucose, but not D-mannitol, stimulation resulted in a rapid and dose-dependent increase in NO release by the cells. However, L-arginine-induced NO release was attenuated significantly in the cells preincubated for more than 12 h with 30 mM D-glucose compared to 10 mM D-glucose. The L-NAME-inhibitable production of nitrite in the media was significantly increased 1.5-2.0-fold until 6 h after incubation with 30 mM D-glucose compared to 10 mM D-glucose. Conclusions We conclude that D-glucose, but not D-mannitol, produces a rapid and dose-dependent increase in NO release, whereas exposure to high D-glucose for more than 12 h may blunt NOS activity and/or NO stability in the GECs. These observations may therefore be important for glomerular endothelial dysfunction induced by hyperglycemia that is still tentative and may have a role in diabetic nephropathy. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:26:55