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Titolo:
Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchuslabrax L.): evidence for intra-molecular induced suppression
Autore:
dos Santos, NMS; Hermsen, T; Rombout, JHWM; Pilstrom, L; Stet, RJM;
Indirizzi:
Univ Wageningen & Res Ctr, Wageningen Inst Anim Sci, Cell Biol & Immunol Grp, NL-6700 AH Wageningen, Netherlands Univ Wageningen & Res Ctr Wageningen Netherlands NL-6700 AH Netherlands Univ Porto, Inst Mol & Cell Biol, P-8234150 Porto, Portugal Univ Porto Porto Portugal P-8234150 Cell Biol, P-8234150 Porto, Portugal Uppsala Univ, Dept Cell & Mol Biol, Immunol Programme, S-75124 Uppsala, Sweden Uppsala Univ Uppsala Sweden S-75124 l Programme, S-75124 Uppsala, Sweden
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 5-6, volume: 25, anno: 2001,
pagine: 387 - 401
SICI:
0145-305X(200106/07)25:5-6<387:ILCVID>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT ONCORHYNCHUS-MYKISS; CYPRINUS-CARPIO L.; SALMO-SALAR L; RAINBOW-TROUT; GENOMIC ORGANIZATION; ANTIBODY-RESPONSES; CHANNEL CATFISH; IMMUNOGLOBULIN; FISH; GENES;
Keywords:
Dicentrarchus labrax; immunoglobulin light chain; expression; variability; DNP494-KLH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Stet, RJM Univ Wageningen & Res Ctr, Wageningen Inst Anim Sci, Cell Biol &Immunol Grp, POB 338, NL-6700 AH Wageningen, Netherlands Univ Wageningen &Res Ctr POB 338 Wageningen Netherlands NL-6700 AH
Citazione:
N.M.S. dos Santos et al., "Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchuslabrax L.): evidence for intra-molecular induced suppression", DEV COMP IM, 25(5-6), 2001, pp. 387-401

Abstract

The coding sequence of the sea bass light chain was obtained by sequentialanchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N = 17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this tow variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation. (C)2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:33:29