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Titolo:
Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks, and affects the movement of water across the small intestinal epitheliumin vitro
Autore:
Kettunen, H; Peuranen, S; Tiihonen, K;
Indirizzi:
Danisco Cultor Innovat Technol Ctr, FIN-02460 Kantvik, Finland Danisco Cultor Innovat Technol Ctr Kantvik Finland FIN-02460 ik, Finland
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 595 - 603
SICI:
1095-6433(200106)129:2-3<595:BAITOO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPORT-SYSTEMS; OSMOTIC-STRESS; CELL-VOLUME; RAT-LIVER; OSMOLYTE; CHANNELS; SPECIFICITY; MACROPHAGES; INDUCTION; GILL;
Keywords:
broiler chick; betaine; osmoregulation; water flux; duodenum; jejunum; epithelium; radio label;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kettunen, H Danisco Cultor Innovat Technol Ctr, Sokeritehtaantie 20, FIN-02460 Kantvik, Finland Danisco Cultor Innovat Technol Ctr Sokeritehtaantie 20 Kantvik Finland FIN-02460
Citazione:
H. Kettunen et al., "Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks, and affects the movement of water across the small intestinal epitheliumin vitro", COMP BIOC A, 129(2-3), 2001, pp. 595-603

Abstract

In Experiment 1, the water holding capacity of broiler chick intestinal tissue was studied in vitro. The chicks were fed with corn-based diets with or without a 0.2% betaine supplementation in the drinking water. Slices fromduodenum and jejunum were incubated in iso-osmotic (300 mM) or hyperosmotic saline (600 mM) with or without 10 mM betaine. The water volume of tissueslices was studied by adding tritiated water in the incubation medium while [C-14]inulin was used to correct for the adherent water. After 30 min of incubation, by which time the steady-state of tritium influx had been achieved, the H-3 and C-14-activities of the tissue slices were measured. The ileal and duodenal tissues incubated in the hyperosmotic saline accumulated less tritium than those incubated in iso-osmotic saline. Duodenal slices incubated in hyperosmotic saline with the presence of betaine showed a tritiumcontent similar to slices incubated in iso-osmotic saline. The data suggest that the presence of betaine helped the duodenal, but not jejunal, epithelium to maintain water balance in hyperosmotic conditions. The dietary betaine supplementation diminished the differences between the incubation treatments in duodenal, but not in ileal tissue. In Experiment 2, the same double labeling method, but with shorter incubation times, was used to assess the rate of water flux from the incubation medium to duodenal or jejunal slices. The dietary treatments las in Experiment 1) had little effect on the results. Betaine in the hyperosmotic saline significantly decreased the rate of tritium accumulation into the tissue slices, indicating that betaine slowed down the influx of water to the epithelium. We suggest that betaine affects the movement of water across the intestinal epithelium and has a role in the osmoregulation of small intestine of broiler chicks. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:48:56