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Titolo:
Selective adhesion, lipid exchange and membrane-fusion processes between vesicles of various sizes bearing complementary molecular recognition groups
Autore:
Marchi-Artzner, V; Gulik-Krzywicki, T; Guedeau-Boudeville, MA; Gosse, C; Sanderson, JM; Dedieu, JC; Lehn, JM;
Indirizzi:
Coll France, Lab Chim Interact Mol, UPR 285, F-75231 Paris 05, France CollFrance Paris France 05 eract Mol, UPR 285, F-75231 Paris 05, France CNRS, Ctr Mol Genet, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 Genet, F-91198 Gif Sur Yvette, France Coll France, Phys Mat Condensee Lab, URA 792, F-75231 Paris 05, France Coll France Paris France 05 ensee Lab, URA 792, F-75231 Paris 05, France Ecole Normale Super, Phys Stat Lab, UMR 8550, F-75005 Paris, France Ecole Normale Super Paris France F-75005 UMR 8550, F-75005 Paris, France
Titolo Testata:
CHEMPHYSCHEM
fascicolo: 6, volume: 2, anno: 2001,
pagine: 367 - 376
SICI:
1439-4235(20010618)2:6<367:SALEAM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMS; AGGREGATION; LIPOSOMES; BILAYERS; SURFACE;
Keywords:
adhesion; aggregation; fusion; molecular recognition; vesicle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Lehn, JM Coll France, Lab Chim Interact Mol, UPR 285, 11 Pl Marcelin Berthelot, F-75231 Paris 05, France Coll France 11 Pl Marcelin Berthelot Paris France 05 05, France
Citazione:
V. Marchi-Artzner et al., "Selective adhesion, lipid exchange and membrane-fusion processes between vesicles of various sizes bearing complementary molecular recognition groups", CHEMPHYSCHE, 2(6), 2001, pp. 367-376

Abstract

Equimolar mixtures of large unilamellar vesicles (LUVs) obtained from mixtures of egg lecithin and lipids containing complementary hydrogen bonding head groups (barbituric acid (BAR) and 2,4,6-triaminopyrimidine (TAP)) were shown to aggregate and fuse. These events have been studied in detail usingelectron microscopy and dynamic light scattering, and by fluorimetry suingmembrane or water-soluble fluorescence probes. I was shown that aggregation was followed by two competitive processes: a) lipid mixing leading to redispersion of the vesicles; b) fusion events generating much larger vesicles. In order to better understand the nature of the interaction, the effects of ionic strength and surface concentration of recognition lipids on the aggregation process were investigated by dynamic light scattering. Additionally, it was possible to inhibit the aggregation kinetics through addition ofa soluble barbitguric acid, competitor. The study was extended to giant unilamellar (v)esicles (GUVs) to investigate the size effect and visualise the; phenomena it; situ. The interactions between complementary LUVs and GUVsor GUVs and GUVs were studied by optical microscopy : using dual fluorescent labelling of both vesicle populations. A selective adhesion of LUVs ontoGUVs was observed by Plectron and optical microscopies, whereas no aggregation took place in case of a GUV/GUV mixture. Furthermore, a fusion assay of GUV and LUV using the difference of size between GUV and LUV and calceineself-quenching showed that no mixing between the aqueous pools occured.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:54:43