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Titolo:
Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in Vitro
Autore:
Jin, X; Mathers, PH; Szabo, G; Katarova, Z; Agmon, A;
Indirizzi:
W Virginia Univ, Dept Neurobiol & Anat, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 & Anat, Morgantown, WV 26506 USA W Virginia Univ, Dept Otolaryngol, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 aryngol, Morgantown, WV 26506 USA W Virginia Univ, Dept Biochem, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 Biochem, Morgantown, WV 26506 USA W Virginia Univ, Sensory Neurosci Res Ctr, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 Res Ctr, Morgantown, WV 26506 USA W Virginia Univ, Grad Program Neurosci, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 eurosci, Morgantown, WV 26506 USA Hungarian Acad Sci, Inst Expt Med, Lab Mol Biol & Genet, H-1450 Budapest, Hungary Hungarian Acad Sci Budapest Hungary H-1450 net, H-1450 Budapest, Hungary
Titolo Testata:
CEREBRAL CORTEX
fascicolo: 7, volume: 11, anno: 2001,
pagine: 666 - 678
SICI:
1047-3211(200107)11:7<666:VBIDTO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMIC-ACID DECARBOXYLASE; RAT VISUAL-CORTEX; ORGANOTYPIC SLICE CULTURES; MOUSE BARREL CORTEX; ACTIVITY-DEPENDENT REGULATION; MONKEY STRIATE CORTEX; SENSORY-MOTOR CORTEX; PRIMARY SOMATOSENSORY CORTEX; LATERAL GENICULATE-NUCLEUS; THALAMIC RETICULAR NUCLEUS;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
134
Recensione:
Indirizzi per estratti:
Indirizzo: Agmon, A W Virginia Univ, Dept Neurobiol & Anat, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 Morgantown, WV 26506 USA
Citazione:
X. Jin et al., "Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in Vitro", CEREB CORT, 11(7), 2001, pp. 666-678

Abstract

The neocortical neuropil has a strong vertical (orthogonal to pia) orientation, constraining the intracortical flow of information and farming the basis for the functional parcellation of the cortex into semi-independent vertical columns or 'modules'. Apical dendrites of excitatory pyramidal neurons are a major component of this Vertical neuropil, but the extent to which inhibitory, GABAergic neurons conform to this structural and functional design is less well documented. We used a gene gun to transfect organotypic slice cultures of meese and rat neocortex with the enhanced green fluorescentprotein (eGFP) gene driven by the promoter for glutamic acid decarboxytase67 (GAD67), an enzyme expressed exclusively in GABAergic cells. Many GAD67-GFP expressing cells were highly fluorescent; and their dendritic morphologies and axonal patterns, revealed in minute detail, were characteristic ofGABAergic neurons. We traced 150 GFP-expressing neurons from confocal image stacks, and estimated the degree of vertical bias in their dendritic trees using a novel computational metric. Over 70% of the neurons in our samplehad dendritic trees with a highly significant vertical bias. We conclude that GABAergic neurons make an important contribution to the vertical neocortical neuropil, and are likely to integrate synaptic inputs from axons terminating within their own module.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 10:38:52