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Titolo:
Enhanced survival of porcine neural xenografts in mice lacking CD1d1, but no effect of NK1.1 depletion
Autore:
Larsson, LC; Anderson, P; Widner, H; Korsgren, O;
Indirizzi:
Univ Lund, Sect Neuronal Survival, Wallenberg Neurosci Ctr, S-22362 Lund, Sweden Univ Lund Lund Sweden S-22362 lenberg Neurosci Ctr, S-22362 Lund, Sweden Univ Uppsala, Rudbeck Lab, Dept Clin Immunol & Transfus Med, S-75185 Uppsala, Sweden Univ Uppsala Uppsala Sweden S-75185 ransfus Med, S-75185 Uppsala, Sweden
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 3, volume: 10, anno: 2001,
pagine: 295 - 304
SICI:
0963-6897(2001)10:3<295:ESOPNX>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; CENTRAL-NERVOUS-SYSTEM; BLOOD-BRAIN-BARRIER; NK T-CELLS; PARKINSONS-DISEASE; NEUROTROPHIC FACTOR; MULTIPLE-SCLEROSIS; IMMUNE-RESPONSES; MICROGLIAL CELLS; TISSUE;
Keywords:
xenotransplantation; NK cells; CD1; Parkinson's disease; brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Larsson, LC Univ Lund, Sect Neuronal Survival, Wallenberg Neurosci Ctr, Solvegatan 17,S-22362 Lund, Sweden Univ Lund Solvegatan 17 Lund Sweden S-22362 2362 Lund, Sweden
Citazione:
L.C. Larsson et al., "Enhanced survival of porcine neural xenografts in mice lacking CD1d1, but no effect of NK1.1 depletion", CELL TRANSP, 10(3), 2001, pp. 295-304

Abstract

Transplantation of embryonic porcine neurons may restore neurological function in patients with Parkinson's disease, if immunological rejection couldbe prevented. This study was performed to investigate the role of natural killer cells (NK cells) and NK1.1+ T cells (NK T cells) in the rejection ofneural xenografts. A cell suspension was prepared from the ventral mesencephalon of 26-27-day-old pig embryos, and 2 mul was implanted in the right striata of mutant CD1d1 null (CD1.1-/-) mice, NK1.1-depleted mice, and controls. The CD1.1-/- mice are deficient in NK T cells and the antigen-presenting molecule CD1d1. Graft survival and host responses were determined immunohistochemically using markers for dopamine neurons, CD4-, CD8- cells, microglia, and macrophages. At 2 weeks, the grafts were significantly larger in CD1.1-/- mice, 0.09 +/- 0.02 mul (mean +/- SEM), compared with controls, 0.05 +/- 0.01 mul. There was no significant difference between NK1.1-depletedmice, 0.02 +/- 0.01 mul, and controls. At 5 weeks, two grafts were still present in the CD1-/- mice, whereas only scars remained in the controls and in the NK1.1-depleted mice, immune reactions were strong at 2 weeks and less pronounced at 5 weeks in all groups. Microglial activation was lower in NK-depleted mice than in the controls at 2 weeks. In contrast to organ xenografting, NK1.1+ cells do not seem to be important mediators of the rejection of discordant cellular neural xenografts. However, our results suggest that the antigen-presenting molecule CD1d1 may be involved in the rejection process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:04:24