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Titolo:
Lack of CD40-dependent B-cell proliferation in B lymphocytes isolated frompatients with persistent polyclonal B-cell lymphocytosis
Autore:
Loembe, MM; Lamoureux, J; Deslauriers, N; Darveau, A; Delage, R;
Indirizzi:
Univ Laval, CREFSIP, Quebec City, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada Univ Laval, GREB, Dept Biochim & Microbiol, Quebec City, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada CHA, Ctr Hematol & Immunol Clin, Quebec City, PQ, Canada CHA Quebec City PQ Canada ematol & Immunol Clin, Quebec City, PQ, Canada
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 3, volume: 113, anno: 2001,
pagine: 699 - 705
SICI:
0007-1048(200106)113:3<699:LOCBPI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; HYPER-IGM SYNDROME; X-LINKED IMMUNODEFICIENCY; LATENT MEMBRANE-PROTEIN-1; TYROSINE KINASE; CD40 RECEPTOR; ACTIVATION; LIGAND; PROTEIN; CD27;
Keywords:
lymphocytosis; B lymphocytes; CD40; TNF-R; B-cell development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Darveau, A Univ Laval, CREFSIP, Pavillon Marchand, Quebec City, PQ G1K 7P4, Canada Univ Laval Pavillon Marchand Quebec City PQ Canada G1K 7P4 nada
Citazione:
M.M. Loembe et al., "Lack of CD40-dependent B-cell proliferation in B lymphocytes isolated frompatients with persistent polyclonal B-cell lymphocytosis", BR J HAEM, 113(3), 2001, pp. 699-705

Abstract

Persistent B-cell lymphocytosis (PPBL) is a haematological disorder diagnosed primarily in adult female smokers that is characterized by a polyclonalincrease in peripheral blood B lymphocytes and a moderate elevation of serum IgM. B lymphocyte-associated cellular abnormalities, such as the occurrence of multi-lobed nuclei, increased bcl2/ Ig gene rearrangements and the identification of an extra long-arm chromosome (i3)(q10) in the B-cell population, indicate that PPBL, could be part of a multi-step process leading tothe emergence of a malignant B Lymphoproliferation. However, the resultingimpact on cellular functional properties remains to be elucidated. Our goal was to address that aspect via the study of B-cell activity following stimulation through CD40, a key molecule of the tumour necrosis factor receptor superfamily involved in B lymphocyte development, In contrast to normal Bcells, PPBL B lymphocytes were unable to respond to the proliferative signal delivered in vitro by CD40, indicating a defect in the CD40 activation pathway. Polymerase chain reaction amplification and sequencing of the receptor as well as FACScan analysis of patient B lymphocytes dismissed the possibility of a defect in either CD40 structure or expression. Moreover, Western blot analysis of tyrosine phosphorylation. an early event in the CD40-signalling cascade, was similar in patients and controls, leading to the conclusion that the defect affecting B lymphocytes in PPBL, patients is probably located downstream of that signalling cascade.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:46:46