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Titolo:
Suppression of a sialyltransferase by antisense DNA reduces invasiveness of human colon cancer cells in vitro
Autore:
Zhu, YT; Srivatana, U; Ullah, A; Gagneja, H; Berenson, CS; Lance, P;
Indirizzi:
Dept Vet Affairs Med Ctr, Dept Med, Buffalo, NY 14215 USA Dept Vet AffairsMed Ctr Buffalo NY USA 14215 Med, Buffalo, NY 14215 USA SUNY Buffalo, Buffalo, NY 14215 USA SUNY Buffalo Buffalo NY USA 14215SUNY Buffalo, Buffalo, NY 14215 USA Buffalo Gen Hosp, Div Gastroenterol E3, Buffalo, NY 14203 USA Buffalo Gen Hosp Buffalo NY USA 14203 roenterol E3, Buffalo, NY 14203 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
fascicolo: 2-3, volume: 1536, anno: 2001,
pagine: 148 - 160
SICI:
0925-4439(20010531)1536:2-3<148:SOASBA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE; SIALIC-ACID; DIFFERENTIAL EXPRESSION; MAACKIA-AMURENSIS; SAMBUCUS-NIGRA; MOLECULAR-CLONING; CARCINOMA-CELLS; E-CADHERIN; BINDING; LECTIN;
Keywords:
colorectal neoplasm; DNA; antisense; neoplasm invasiveness; sialyltransferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lance, P Dept Vet Affairs Med Ctr, Dept Med, Buffalo, NY 14215 USA Dept Vet Affairs Med Ctr Buffalo NY USA 14215 falo, NY 14215 USA
Citazione:
Y.T. Zhu et al., "Suppression of a sialyltransferase by antisense DNA reduces invasiveness of human colon cancer cells in vitro", BBA-MOL BAS, 1536(2-3), 2001, pp. 148-160

Abstract

Transfer of terminal alpha2,6-linked sialic acids to N-glycans is catalyzed by beta -galactoside alpha2,6-sialyltransferase (ST6Gal I). Expression ofSTGGal I and its products is reportedly increased in colon cancers. To investigate directly the functional effects of ST6Gal I expression, human colon cancer (HT29) cells were transfected with specific antisense DNA. ST6Gal I mRNA and protein were virtually undetectable in six strains of transfected HT29 cells. STGGal activity was reduced to 14% of control (P<0.005) in transfected cells. Expression of terminal <alpha>2,6- and alpha2,3-linked sialic acids, and unmasked N-acetyllactosamine oligosaccharides, respectively,was assessed using flow cytometry and fluoresceinated Sambucus nigra, Maackia amurensis and Erythrina cristagalli lectins. Results indicated a major reduction in expression of alpha2,6-linked sialic acids and counterbalancing increase in unmasked N-acetyllactosamines in antisense DNA-transfected cells, without altered expression of alpha2,3-linked sialic acids or ganglioside profiles. The ability of transfected cells to form colonies in soft agar and to invade extracellular matrix material (Matrigel), respectively, in vitro was reduced by approx. 98% (P < 0.0001) and more than 3-fold (P < 0.005) compared to parental HT29 cells. These results indicate that N-glycans bearing terminal alpha2,6-linked sialic acids may enhance the invasive potential of colon cancer cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 03:40:32