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Titolo:
Novel forms of squamous cell carcinoma antigen transcripts produced by alternative splicing
Autore:
Suminami, Y; Kishi, F; Murakami, A; Sakaguchi, Y; Nawata, S; Numa, F; Kato, H;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Obstet & Gynecol, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Yamaguchi Univ, Genet Res Inst, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1-2, volume: 1519, anno: 2001,
pagine: 122 - 126
SICI:
0167-4781(20010528)1519:1-2<122:NFOSCC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERINE PROTEINASE-INHIBITOR; CYSTEINE PROTEINASES; CRYSTAL-STRUCTURE; SCC ANTIGEN-1; SERPIN; CHYMOTRYPSIN; EXPRESSION; GENE;
Keywords:
squamous cell carcinoma antigen; squamous cell carcinoma antigen 1b; squamous cell carcinoma antigen 2b; serpin; alternative splicing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Suminami, Y Yamaguchi Univ, Sch Med, Dept Obstet & Gynecol, 1-1-1 Minamikogushi, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ 1-1-1 Minamikogushi UbeYamaguchi Japan 7558505
Citazione:
Y. Suminami et al., "Novel forms of squamous cell carcinoma antigen transcripts produced by alternative splicing", BBA-GENE ST, 1519(1-2), 2001, pp. 122-126

Abstract

Squamous cell carcinoma antigen (SCCA) is a member of the ovalbumin serineprotease inhibitor family, and the serum level of SCCA is a tumor marker of squamous cell carcinoma. Reverse transcription (RT)-PCR of the squamous cell carcinoma cell line showed the existence of a 156 base shorter transcript compared with that of SCCA1 cDNA. By inverse PCR. we cloned the full length cDNA of this SCCA (SCCA1b). Sequence analysis of the complete 1541 bp SCCA1b cDNA showed that it coded for 338 amino acids and had no typical signal sequence in the NH2 terminus. The cDNA was expressed in Escherichia coliand the product was detected using Western blotting with antibodies against SCCA. Furthermore, RT-PCR of the full coding region of SCCA2 cDNA from cancer tissue showed the existence of a 63 base short transcript (SCCA2b). A comparison of SCCA1b and SCCA2b cDNA with the SCCA1 and SCCA2 genes showed that these messages were derived from each gene by an alternative splicing mechanism. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:12:42