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Titolo:
Effect of cholesterol on miscibility and phase behavior in binary mixtureswith synthetic ceramide 2 and octadecanoic acid. Infrared studies
Autore:
Chen, HC; Mendelsohn, R; Rerek, ME; Moore, DJ;
Indirizzi:
Rutgers State Univ, Dept Chem, Newark, NJ 07102 USA Rutgers State Univ Newark NJ USA 07102 v, Dept Chem, Newark, NJ 07102 USA Int Specialty Prod, Wayne, NJ 07470 USA Int Specialty Prod Wayne NJ USA 07470 Specialty Prod, Wayne, NJ 07470 USA Unilever Res US, Edgewater, NJ 07020 USA Unilever Res US Edgewater NJ USA07020 er Res US, Edgewater, NJ 07020 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 2, volume: 1512, anno: 2001,
pagine: 345 - 356
SICI:
0005-2736(20010606)1512:2<345:EOCOMA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MODEL STRATUM-CORNEUM; EPIDERMAL PERMEABILITY BARRIER; FT-RAMAN SPECTROSCOPY; X-RAY-DIFFRACTION; CONFORMATIONAL ORDER; LIPID DOMAINS; HUMAN ERYTHROCYTES; MEMBRANE APPROACH; CELL-SHAPE; SKIN;
Keywords:
ceramide; stratum corneum; lipid barrier; domain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Mendelsohn, R Rutgers State Univ, Dept Chem, Warren St, Newark, NJ 07102 USA Rutgers State Univ Warren St Newark NJ USA 07102 J 07102 USA
Citazione:
H.C. Chen et al., "Effect of cholesterol on miscibility and phase behavior in binary mixtureswith synthetic ceramide 2 and octadecanoic acid. Infrared studies", BBA-BIOMEMB, 1512(2), 2001, pp. 345-356

Abstract

The three main lipid components of the stratum corneum, namely ceramides, free fatty acids and cholesterol, play a fundamental role in the maintenance of the skin barrier. The current investigation is aimed toward understanding the miscibility and intermolecular interactions of these lipids. Towardthis end, Fourier transform infrared spectroscopic studies of the three possible equimolar binary mixtures of cholesterol, a synthetic non-hydroxylated fatty acid N-acyl sphingosine with a C18 chain length (N-stearoylsphingosine, approximating human ceramide 2), and stearic acid were undertaken. The thermotropic responses of the methylene stretching and scissoring vibrations were used to evaluate chain conformation and packing respectively. Selective perdeuteration, of either the stearic acid or the ceramide acid chains, permitted separate and simultaneous evaluation of the conformational order and packing properties of the sphingosine chain, the amide linked fatty acid chains and/or the stearic acid chain. Whereas cholesterol mixed well with ceramide at physiological temperatures, the stearic acid was miscible with the cholesterol only at relatively high temperatures where the fatty acid is disordered. A complex interaction between stearic acid and ceramide was detected. A separate fatty acid-rich phase persisted until at least 50 degreesC, whereas at higher temperatures the components appear to be quite miscible. However, a preferential association of the fatty acid with the ceramide base chain is indicated. None of the binary systems studied exhibit miscibility and interactions resembling those in the ternary mixtures of these substances, which is widely used to model stratum corneum. The role of cholesterol in controlling the miscibility characteristics in the ternary system is evident. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 26/01/21 alle ore 02:59:53